Analysis of low molecular weight GTPase activity in endothelial cell cultures

被引：14

作者：

Wittchen, Erika S. ^{[1
]}

Burridge, Keith ^{[1
]}

机构：

[1] Univ N Carolina, Lineberger Comprehens Canc Ctr, Dept Cell & Dev Biol, Chapel Hill, NC 27599 USA

来源：

ANGIOGENESIS: IN VITRO SYSTEMS | 2008年 / 443卷

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/S0076-6879(08)02014-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

GTPases control a myriad of cellular processes, including cell migration, proliferation, polarity, and cell adhesion, which includes cell-cell junction regulation. Angiogenesis requires many of these diverse cellular events to occur appropriately. In this chapter, we describe the techniques for in vitro assessment of GTPase activity in endothelial cells grown in monolayer culture as a model system for studying their role during angiogenesis.

引用

收藏

页码：285 / 298

页数：14

相关论文

共 21 条

[1] Involvement of RhoA/Rho kinase signaling in VEGF-induced endothelial cell migration and angiogenesis in vitro [J].

Amerongen, GPV ;

Koolwijk, P ;

Versteilen, A ;

van Hinsbergh, VWM .

ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, 2003, 23 (02) :211-217

[2] Rap1 promotes cell spreading by localizing Rac guanine nucleoticle exchange factors [J].

Arthur, WT ;

Quilliam, LA ;

Cooper, JA .

JOURNAL OF CELL BIOLOGY, 2004, 167 (01) :111-122

[3] Role of Rho GTPases in thrombin-induced lung vascular endothelial cells barrier dysfunction [J].

Birukova, AA ;

Smurova, K ;

Birukov, KG ;

Kaibuchi, K ;

Garcia, JGN ;

Verin, AD .

MICROVASCULAR RESEARCH, 2004, 67 (01) :64-77

[4] Rac regulates endothelial morphogenesis and capillary assembly [J].

Connolly, JO ;

Simpson, N ;

Hewlett, L ;

Hall, A .

MOLECULAR BIOLOGY OF THE CELL, 2002, 13 (07) :2474-2485

[5] Regulation of vascular endothelial barrier function by Epac, a cAMP-activated exchange factor for Rap GTPase [J].

Cullere, X ;

Shaw, SK ;

Andersson, L ;

Hirahashi, J ;

Luscinskas, FW ;

Mayadas, TN .

BLOOD, 2005, 105 (05) :1950-1955

[6] A novel Epac-specific cAMP analogue demonstrates independent regulation of Rap1 and ERK [J].

Enserink, JM ;

Christensen, AE ;

de Rooij, J ;

van Triest, M ;

Schwede, F ;

Genieser, HG ;

Doskeland, SO ;

Blank, JL ;

Bos, JL .

NATURE CELL BIOLOGY, 2002, 4 (11) :901-906

[7] Rapid Ca2+-mediated activation of Rap1 in human platelets [J].

Franke, B ;

Akkerman, JWN ;

Bos, JL .

EMBO JOURNAL, 1997, 16 (02) :252-259

[8] Inhibition of GTPase activating protein stimulation of Ras-p21 GTPase by the Krev-1 gene product [J].

Frech, M ;

John, J ;

Pizon, V ;

Chardin, P ;

Tavitian, A ;

Clark, R ;

Mccormick, F ;

Wittinghofer, A .

SCIENCE, 1990, 249 (4965) :169-171

[9] VEGF-induced Rac1 activation in endothelial cells is regulated by the guanine nucleotide exchange factor Vav2 [J].

Garrett, Tiana A. ;

van Buula, Jaap D. ;

Burridge, Keith .

EXPERIMENTAL CELL RESEARCH, 2007, 313 (15) :3285-3297

[10] Endothelial tubes assemble from intracellular vacuoles in vivo [J].

Kamei, Makoto ;

Saunders, W. Brian ;

Bayless, Kayla J. ;

Dye, Louis ;

Davis, George E. ;

Weinstein, Brant M. .

NATURE, 2006, 442 (7101) :453-456