Centrifugation does not alter spatial distribution of 'BEP4' mRNA in paracentrotus lividus EGG

Paracentrotus lividus unfertilized eggs were centrifuged in a sucrose gradient, so to split each into two parts: a nucleated light fragment and an anucleated heavy fragment. Northern blot analyses utilizing a bep4 probe as animal marker and H2A histone gene and 12S-mit RNA as controls indicate that the eggs are elongated along the animal-vegetal axis during centrifugation and thereafter split into an animal and a vegetal half, Treatment of the eggs with colchicine before centrifugation abolishes the animal localization of bep4 mRNA. (C) 1997 Federation of European Biochemical Societies.