Super-resolution imaging in live Caulobacter crescentus cells using photoswitchable EYFP

被引：260

作者：

Biteen, Julie S. ^{[1
]}

Thompson, Michael A. ^{[1
]}

Tselentis, Nicole K. ^{[1
]}

Bowman, Grant R. ^{[2
]}

Shapiro, Lucy ^{[2
]}

Moerner, W. E. ^{[1
]}

机构：

[1] Stanford Univ, Dept Chem, Stanford, CA 94305 USA

[2] Stanford Univ, Dept Dev Biol, Stanford, CA 94305 USA

来源：

NATURE METHODS | 2008年 / 5卷 / 11期

基金：

美国国家科学基金会; 美国国家卫生研究院;

关键词：

D O I：

10.1038/nmeth.1258

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The commonly used, monomeric EYFP enabled imaging of intracellular protein structures beyond the optical resolution limit ('super-resolution' imaging) in living cells. By combining photoinduced activation of single EYFP fusions and time-lapse imaging, we obtained sub-40 nm resolution images of the filamentous superstructure of the bacterial actin protein MreB in live Caulobacter crescentus cells. These studies demonstrated that EYFP is a useful emitter for in vivo super-resolution imaging.

引用

页码：947 / 949

页数：3

共 15 条

[1] PROPOSED METHOD FOR MOLECULAR OPTICAL IMAGING
BETZIG, E
[J]. OPTICS LETTERS, 1995, 20 (03) : 237 - 239
[2] Imaging intracellular fluorescent proteins at nanometer resolution
Betzig, Eric
Patterson, George H.
Sougrat, Rachid
Lindwasser, O. Wolf
Olenych, Scott
Bonifacino, Juan S.
Davidson, Michael W.
Lippincott-Schwartz, Jennifer
Hess, Harald F.
[J]. SCIENCE, 2006, 313 (5793) : 1642 - 1645
[3] The bacterial cytoskeleton:: In vivo dynamics of the actin-like protein Mbl of Bacillus subtilis
Carballido-López, R
Errington, J
[J]. DEVELOPMENTAL CELL, 2003, 4 (01) : 19 - 28
[4] On/off blinking and switching behaviour of single molecules of green fluorescent protein
Dickson, RM
Cubitt, AB
Tsien, RY
Moerner, WE
[J]. NATURE, 1997, 388 (6640) : 355 - 358
[5] MreB, the cell shape-determining bacterial actin homologue, co-ordinates cell wall morphogenesis in Caulobacter crescentus
Figge, RM
Divakaruni, AV
Gober, JW
[J]. MOLECULAR MICROBIOLOGY, 2004, 51 (05) : 1321 - 1332
[6] Resolution of λ/10 in fluorescence microscopy using fast single molecule photo-switching
Geisler, C.
Schoenle, A.
von Middendorff, C.
Bock, H.
Eggeling, C.
Egner, A.
Hell, S. W.
[J]. APPLIED PHYSICS A-MATERIALS SCIENCE & PROCESSING, 2007, 88 (02): : 223 - 226
[7] An actin-like gene can determine cell polarity in bacteria
Gitai, Z
Dye, N
Shapiro, L
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (23) : 8643 - 8648
[8] Ultra-high resolution imaging by fluorescence photoactivation localization microscopy
Hess, Samuel T.
Girirajan, Thanu P. K.
Mason, Michael D.
[J]. BIOPHYSICAL JOURNAL, 2006, 91 (11) : 4258 - 4272
[9] Single molecules of the bacterial actin MreB undergo directed treadmilling motion in Caulobacter crescentus
Kim, So Yeon
Gitai, Zemer
Kinkhabwala, Anika
Shapiro, Lucy
Moerner, W. E.
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (29) : 10929 - 10934
[10] Single-molecule mountains yield nanoscale cell images
Moerner, W. E.
[J]. NATURE METHODS, 2006, 3 (10) : 781 - 782

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