Crystallization and preliminary crystallographic analysis of a novel cytochrome P450 from Mycobacterium tuberculosis

被引：9

作者：

Mowat, CG

Leys, D

McLean, KJ

Rivers, SL

Richmond, A

Munro, AW

Lombardia, MO

Alzari, PM

Reid, GA

Stephen, KCB

Walkinshaw, MD

机构：

[1] Univ Edinburgh, Inst Cell & Mol Biol, Edinburgh EH9 3JR, Midlothian, Scotland

[2] Univ Edinburgh, Dept Chem, Edinburgh EH9 3JJ, Midlothian, Scotland

[3] Univ Leicester, Dept Biochem, Leicester LE1 7RH, Leics, England

[4] Univ Strathclyde, Royal Coll, Dept Pure & Appl Chem, Glasgow G1 1XL, Lanark, Scotland

[5] Inst Pasteur, Struct Biochem Unit, F-75724 Paris, France

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY | 2002年 / 58卷

关键词：

D O I：

10.1107/S0907444902002676

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The product of the Rv2276 gene of Mycobacterium tuberculosis is a cytochrome P450 (P450 MT2, CYP121) which has been shown to bind tightly to a range of azole-based antifungal drugs (e.g. miconazole, clotrimazole). These drugs are potent inhibitors of mycobacterial growth, suggesting that P450 MT2 (CYP121) may be a potential drug target. The enzyme has been overexpressed in Escherichia coli and crystallized by the hanging-drop method. Crystals of P450 MT2 (CYP121) belong to the hexagonal space group P6(1)22 or P6(5)22, with unit-cell parameters a = b = 78.3, c = 265.6 Angstrom. Native data have been collected to 1.6 Angstrom resolution and Hg-derivative data to 2.5 Angstrom resolution using a synchrotron-radiation source.

引用

页码：704 / 705

页数：2

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