Mobilization of a retrotransposon in 5-azacytidine-treated fungus Fusarium oxysporum

被引：3

作者：

Akiyama, Kouichi ^{[1
]}

Katakami, Hatsue ^{[2
]}

Takata, Renkichi ^{[2
]}

机构：

[1] Ehime Univ, Integrated Ctr Sci, Satellite Venture Business Lab, Matsuyama, Ehime 7908566, Japan

[2] Ehime Univ, Fac Agr, Matsuyama, Ehime 7908566, Japan

来源：

PLANT BIOTECHNOLOGY | 2007年 / 24卷 / 03期

关键词：

5-azacytidine; Foxy; Fusarium oxysporum; retrotransposon; transposition;

D O I：

10.5511/plantbiotechnology.24.345

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

It is well known that DNA methylation is involved in the control of transposable elements in eukaryotic cells. Recent studies indicate that demethylation of DNA in a mutant of Chlamydomonas and Arabidopsis causes transcriptional activation and mobilization of transposons. In this report, transposition of a retrotransposon was investigated in the phytopathogenic fungus Fusarium oxysporum treated with 5-azacytidine (5azaC), a reagent that causes reduction in the DNA methylation level. The results showed elevated transposition frequency in 5azaC-treated isolates when they were incubated for a long time. However, increase of retrotransposon transcripts was not observed, suggesting that the retrotransposon was mobilized by a mechanism other than its transcriptional activation.

引用

页码：345 / 348

页数：4

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