Mechanism of [Ca2+](i) increase by extracellular ATP in isolated rabbit renal proximal tubules

被引:31
作者
Yamada, H
Seki, G
Taniguchi, S
Uwatoko, S
Suzuki, K
Kurokawa, K
机构
[1] UNIV TOKYO, SCH MED, DEPT INTERNAL MED 1, BUNKYO KU, TOKYO 113, JAPAN
[2] TOKYO UNIV AGR & TECHNOL, HLTH SERV CTR, FUCHU, TOKYO 183, JAPAN
来源
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY | 1996年 / 270卷 / 04期
关键词
purinergic receptor; fura; 2; microelectrodes; proximal tubular cells;
D O I
10.1152/ajpcell.1996.270.4.C1096
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The effects of extracellular ATP on cytosolic Ca2+ concentrations ([Ca2+](i)) and cell membrane potentials (V-b) of rabbit renal proximal tubules were investigated using fura 2 and microelectrodes. ATP transiently increased [Ca2+](i) without an apparent sustained phase, and the maximum effect was obtained at 10 mu M. ADP, adenosine 5'-O-(3-thiotriphosphate), and 2-methylthioadenosine 5'-triphosphate were equally effective as ATP, whereas UTP, adenosine, and alpha beta-methyleneadenosine 5'-triphosphate were far less effective. The [Ca2+](i) responses to ATP were strongly inhibited by reactive blue 2, a P-2-purinergic receptor antagonist. The removal of extracellular Ca2+ as well as the addition of thapsigargin also markedly attenuated the responses to ATP. In addition, ATP had virtually no effect on V-b, except for the occasional small depolarization by 300 mu M ATP. These results indicate that extracellular ATP increases [Ca2+](i) through a P-2-purinergic receptor and that this effect of ATP is dependent on both extracellular and intracellular Ca2+.
引用
收藏
页码:C1096 / C1104
页数:9
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