Mechanisms involved in the resistance of Enterococcus faecalis to calcium hydroxide

被引:339
作者
Evans, M
Davies, JK
Sundqvist, G
Figdor, D
机构
[1] Univ Melbourne, Sch Dent Sci, Melbourne, Vic 3000, Australia
[2] Monash Univ, Dept Microbiol, Melbourne, Vic 3004, Australia
[3] Umea Univ, Dept Endodont, Umea, Sweden
关键词
E; faecalis; proton pump; sodium hypochlorite; stress response;
D O I
10.1046/j.1365-2591.2002.00504.x
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
Aim This study sought to clarify the mechanisms that enable E. faecalis to survive the high pH of calcium hydroxide. Methodology E. faecalis strain JH2-2 was exposed to sublethal concentrations of calcium hydroxide, with and without various pretreatments. Blocking agents were added to determine the role of stress-induced protein synthesis and the cell wall-associated proton pump. Results F. faecalis was resistant to calcium hydroxide at a pH of 11.1, but not pH 11.5. Pre-treatment with calcium hydroxide pH 10.3 induced no tolerance to further exposure at pH 11.5. No difference in cell survival was observed when protein synthesis was blocked during stress induction. however, addition of a proton pump inhibitor resulted in a dramatic reduction of cell viability of E. faecalis in calcium hydroxide. Conclusions Survival of F. faecalis in calcium hydroxide appears to be unrelated to stress induced protein synthesis, but a functioning proton pump is critical for survival of E. faecalis at high pH.
引用
收藏
页码:221 / 228
页数:8
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