Metabolism of 24-EPI-castasterone and 24-EPI-brassinolide in cell suspension cultures of Ornithopus sativus

被引：38

作者：

Kolbe, A ^{[1
]}

Schneider, B ^{[1
]}

Porzel, A ^{[1
]}

Adam, G ^{[1
]}

机构：

[1] INST BIOCHEM PFLANZEN, D-06018 HALLE, GERMANY

来源：

PHYTOCHEMISTRY | 1996年 / 41卷 / 01期

关键词：

Ornithopus sativus; leguminosae; cell suspension cultures; metabolism; tritium-labelled brassinosteroids; 24-epi-brassinolide; 24-epi-castasterone; 20-keto-pregnane; hydroxylation; catabolism;

D O I：

10.1016/0031-9422(95)00546-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Exogenously applied 24-epi-brassinolide and 24-epi-castasterone are transformed by cell suspension cultures of Ornithopus sativus in a multistep pathway to give 20-keto-pregnane derivatives. As intermediates of these degradations 20R-hydroxy-3,24-bisepi-brassinolide and 20R-hydroxy-3,24-bisepi-castasterone, respectively, as well as 2 alpha, 3 beta-dihydroxy-5 alpha-pregnane-6,20-dione were isolated from the culture medium and their structures were elucidated by means of H-1 NMR and mass spectrometry. In addition to side-chain cleavage, 25-hydroxylation to give 25-hydroxy-3,24-bisepi-brassinolide was also observed.

引用

页码：163 / 167

页数：5

共 13 条

[1] CATABOLISM OF CRUSTECDYSONE IN BLOWFLY CALLIPHORA STYGIA [J].