Cooperative assembly of EBNA1 on the Epstein-Barr virus latent origin of replication

被引：44

作者：

Summers, H

Barwell, JA

Pfuetzner, RA

Edwards, AM

Frappier, L

机构：

[1] MCMASTER UNIV,DEPT PATHOL,INST MOLEC BIOL & BIOTECHNOL,CANC RES GRP,HAMILTON,ON L8N 3Z5,CANADA

[2] MCMASTER UNIV,DEPT BIOCHEM,INST MOLEC BIOL & BIOTECHNOL,CANC RES GRP,HAMILTON,ON L8N 3Z5,CANADA

来源：

JOURNAL OF VIROLOGY | 1996年 / 70卷 / 02期

关键词：

D O I：

10.1128/JVI.70.2.1228-1231.1996

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The EBNA1 protein of Epstein-Barr virus (EBV) activates DNA replication by binding to multiple copies of its 18-bp recognition sequence present in the Epstein-Barr virus latent origin of DNA replication, oriP. Using electrophoretic mobility shift assays, we have localized the minimal DNA binding domain of EBNA1 to between amino acids 470 and 607. We have also demonstrated that EBNA1 assembles cooperatively on the dyad symmetry subelement of oriP and that this cooperative interaction is mediated by residues within the minimal DNA binding and dimerization domain of EBNA1.

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页码：1228 / 1231

页数：4

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