Primary and secondary infections of Macaca fascicularis monkeys with Asian and American genotypes of dengue virus 2

被引：31

作者：

Bernardo, Lidice ^{[1
]}

Izquierdo, Alienys ^{[1
]}

Prado, Irina ^{[1
]}

Rosario, Delfina ^{[1
]}

Alvarez, Mayling ^{[1
]}

Santana, Emidalys ^{[1
]}

Castro, Jorge ^{[2
]}

Martinez, Rafael

Rodriguez, Rosmari ^{[1
]}

Morier, Luis ^{[1
]}

Guillen, Gerardo ^{[2
]}

Guzman, Maria G. ^{[1
]}

机构：

[1] Pedro Kouri Trop Med Inst IPK, Dept Virol, PAHO WHO Collaborating Ctr Study Dengue & Its Vec, Havana, Cuba

[2] Int Ctr Genet Engn & Biotechnol, Havana 10600, Cuba

来源：

CLINICAL AND VACCINE IMMUNOLOGY | 2008年 / 15卷 / 03期

关键词：

D O I：

10.1128/CVI.00208-07

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The goal of this study was to compare the immune response and the protection capacity induced by the dengue virus 2 (DENV-2) American and Asian genotypes in Macaca fascicularis monkeys. Animals were infected with American or Asian DENV-2 strains and challenged 1 year later with a DENV-2 Asian genotype strain. The viremia and monkey antibody levels were similar for the different strains after primary and secondary infection; however, the functionality of the antibody response was different. A limited viral replication was demonstrated after the secondary infection in all the monkeys. No virus was isolated in tissue culture, while reverse transcription-PCR showed a late positive reaction in four of five challenged monkeys. The immunoglobulin M response pattern and the detection of antibodies to specific proteins by Western blotting supported the protection data. Despite the demonstration of the protective effect after homologous challenge, a strong anamnestic antibody response was observed.

引用

页码：439 / 446

页数：8

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