Investigation of substance P transport across the blood-brain barrier

被引：39

作者：

Freed, AL ^{[1
]}

Audus, KL ^{[1
]}

Lunte, SM ^{[1
]}

机构：

[1] Univ Kansas, Sch Pharm, Dept Chem Pharmacol, Lawrence, KS 66047 USA

来源：

PEPTIDES | 2002年 / 23卷 / 01期

基金：

美国国家科学基金会;

关键词：

substance P; transport; blood-brain barrier; brain microvessel endothelial cells; capillary electrophoresis; laser induced fluorescence detection;

D O I：

10.1016/S0196-9781(01)00592-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The transport of substance P (SP) was investigated using the bovine brain microvessel endothelial cell culture model of the blood-brain barrier (BBB). The samples were derivatized precolumn with naphthalene dialdehyde, then analyzed by cyclodextrin-modified micellar electrokinetic chromatography with laser-induced fluorescence detection. SP crossed the BBB in both the apical-to-basolateral and basolateral-to-apical directions through an active transport mechanism. The transport of SP from the apical side was demonstrated to be via transcytosis. The N-terminal (SP1-4) and C-terminal (SP3-11) fragments were also found to permeate the BBB from the apical side. (C) 2002 Elsevier Science Inc. All rights reserved.

引用

页码：157 / 165

页数：9

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