Structure of wyosine, the condensed tricyclic nucleoside of torula yeast phenylalanine transfer ribonucleic acid

被引：8

作者：

Itaya, T ^{[1
]}

Kanai, T ^{[1
]}

Sawada, T ^{[1
]}

机构：

[1] Kanazawa Univ, Fac Pharmaceut Sci, Kanazawa, Ishikawa 920, Japan

来源：

CHEMICAL & PHARMACEUTICAL BULLETIN | 2002年 / 50卷 / 04期

关键词：

wyosine; minor nucleoside; torula yeast tRNA(Phe); fluorescent nucleoside; condensed tricyclic nucleoside; H-1-NMR;

D O I：

10.1248/cpb.50.547

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Large-scale isolation of the minor nucleoside wyosine of torula yeast tRNA(Phe) was accomplished by a combination of enzymatic digestion and reversed-phase chromatography: the wyosine-containing nucleotide fraction, which was obtained by partial digestion of unfractionated tRNA (1 g) with nuclease P-1, was concentrated by reversed-phase column chromatography followed by complete digestion with nuclease P-1/alkaline phosphatase. The nucleoside mixture thus obtained was purified by reversed-phase HPLC, providing wyosine (70 mug). Comparison of this nucleoside with a chemically synthesized authentic sample has unambiguously established that the structure of wyosine is 4,6-dimethyl-3-beta-D-ribofuranosyl-3,4-dihydro-9H-imidazo[1,2-a]purin-9-one (2).

引用

页码：547 / 548

页数：2

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