Flow cytometry analysis of platelet cyclooxygenase-2 expression:: induction of platelet cyclooxygenase-2 in patients undergoing coronary artery bypass grafting

被引：50

作者：

Weber, AA

Przytulski, B

Schumacher, M

Zimmermann, N

Gams, E

Hohlfeld, T

Schrör, K

机构：

[1] Univ Klinikum Dusseldorf, Inst Pharmakol & Klin Pharmakol, Dusseldorf, Germany

[2] Univ Klinikum Dusseldorf, Klin Thorax & Kardiovask Chirurg, Dusseldorf, Germany

来源：

BRITISH JOURNAL OF HAEMATOLOGY | 2002年 / 117卷 / 02期

关键词：

platelets; cyclooxygenase-1; cyclooxygenase-2; flow cytometry; coronary artery bypass surgery;

D O I：

10.1046/j.1365-2141.2002.03423.x

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

There are conflicting reports about the expression of cyclooxygenase (COX)-2 in human platelets. The present study describes a flow cytometric method for the measurement of platelet COX. Both COX-1 and COX-2 were shown to be expressed in platelets from patients undergoing a coronary artery bypass graft. There was a significant increase in COX-2 expression at day 5 as compared with pre-surgery values (mean fluorescence 12.31 +/- 0.88 versus 9.15 +/- 0.88; means +/- SEM, n = 7, P < 0.05), whereas COX-1 levels did not change (13.45 +/- 1.11 versus 12.38 +/- 1.41; n = 7, P > 0.05).

引用

页码：424 / 426

页数：3

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