Ascorbate oxidase from starfruit (Averrhoa carambola):: preparation and its application in the determination of ascorbic acid from fruit juices

被引：37

作者：

bin Saari, N ^{[1
]}

Osman, A

Selamat, J

Fujita, S

机构：

[1] Univ Putra Malaysia, Fac Food Sci & Biotechnol, Dept Food Sci, Serdang 43400, Malaysia

[2] Saga Univ, Fac Agr, Food Sci Lab, Saga 840, Japan

来源：

FOOD CHEMISTRY | 1999年 / 66卷 / 01期

关键词：

ascorbate oxidase; ascorbic acid; starfruit; averrhoa carambola;

D O I：

10.1016/S0308-8146(98)00170-8

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

A study was conducted to utilize ascorbate oxidase (AAO) from very immature starfruit for the enzymatic determination of ascorbic acid (AsA) in colored samples. The enzyme preparation was carried out by a combination of (NH4)(2)SO4 fractionation, DEAE-Toyopearl 650 M and ultrafiltration. A calibration curve for AsA was constructed by plotting the amount of AsA oxidized by the enzyme at a specified reaction time against the absorbance. The curve showed a linear relationship in the range of 0-100 mu g ml(-1) AsA used. Using the plot, the values of AsA in juice samples were determined and compared with the conventional 2,6-dichloroindophenol method. (C) 1999 Elsevier Science Ltd. All rights reserved.

引用

页码：57 / 61

页数：5

共 13 条

[1] *ASS OFF AN CHEM, 1975, OFF METH AN, P829
[2] DETERMINATION OF PROTEIN - MODIFICATION OF LOWRY METHOD THAT GIVES A LINEAR PHOTOMETRIC RESPONSE
HARTREE, EF
[J]. ANALYTICAL BIOCHEMISTRY, 1972, 48 (02) : 422 - &
[3] LEE MH, 1973, J BIOL CHEM, V248, P6596
[4] EXPRESSION OF ASCORBIC-ACID OXIDASE IN ZUCCHINI SQUASH (CUCURBITA-PEPO L)
LIN, LS
VARNER, JE
[J]. PLANT PHYSIOLOGY, 1991, 96 (01) : 159 - 165
[5] TEMPERATURE, PH AND UV IRRADIATION EFFECTS ON ASCORBATE OXIDASE
MACCARRONE, M
DANDREA, G
SALUCCI, ML
AVIGLIANO, L
FINAZZIAGRO, A
[J]. PHYTOCHEMISTRY, 1993, 32 (04) : 795 - 798
[6] ISOLATION AND PURIFICATION OF ASCORBATE OXIDASE FROM ACREMONIUM SP HI-25
MURAO, S
ITOH, H
YAJIMA, T
OZAKI, Y
FUKUYASU, S
SHIN, T
[J]. BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 1992, 56 (06) : 847 - 852
[7] Saari N., 1996, FOOD SCI TECHNOLOGY, V2, P154
[8] SAARI NB, 1995, J FOOD BIOCHEM, V19, P321
[9] SEKIYA J, 1990, SOIL SCI PLANT NUTR, V36, P1, DOI 10.1080/00380768.1990.10415704
[10] DEVELOPMENT OF ASCORBATE OXIDASE ACTIVITY AND ITS ISOENZYME PATTERN IN ROOTS OF PEA-SEEDLINGS
SUZUKI, Y
OGISO, K
[J]. PHYSIOLOGIA PLANTARUM, 1973, 29 (02) : 169 - 172

← 1 2 →