Crystallization and preliminary crystallographic studies of dimeric disintegrins from the venom of two Agkistrodon snakes

被引：8

作者：

Fujii, Y

Okuda, D

Fujimoto, Z

Morita, T

Mizuno, H

机构：

[1] Natl Inst Agrobiol Sci, Dept Biochem, Tsukuba, Ibaraki 3058602, Japan

[2] Meiji Pharmaceut Univ, Dept Biochem, Tokyo 2048588, Japan

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY | 2002年 / 58卷

关键词：

D O I：

10.1107/S090744490101736X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Piscivostatin (PVS) and acostatin are members of the disintegrin family of platelet-aggregation inhibitors found in snake venoms and are dimeric disintegrins which play different roles to the monomeric disintegrins. The crystals of PVS belonged to the P2(1)2(1)2(1) space group, with unit-cell parameters a = 34.3, b = 54.4, c = 115.9 Angstrom, and diffracted to 2.0 Angstrom resolution. The crystals of acostatin belonged to the P2(1) space group, with unit-cell parameters a = 55.4, b = 69.3, c = 63.5 Angstrom, beta = 111.7 degrees, and diffracted to 2.8 Angstrom resolution.

引用

收藏

页码：145 / 147

页数：3

相关论文

共 16 条

[1] A COMMON PRECURSOR FOR A PUTATIVE HEMORRHAGIC PROTEIN AND RHODOSTOMIN, A PLATELET-AGGREGATION INHIBITOR OF THE VENOM OF CALLOSELASMA-RHODOSTOMA - MOLECULAR-CLONING AND SEQUENCE-ANALYSIS [J].

AU, LC ;

HUANG, YB ;

HUANG, TF ;

TEH, GW ;

LIN, HH ;

CHOO, KB .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1991, 181 (02) :585-593

[2] HEMORRHAGIC METALLOPROTEINASES FROM SNAKE-VENOMS [J].

BJARNASON, JB ;

FOX, JW .

PHARMACOLOGY & THERAPEUTICS, 1994, 62 (03) :325-372

[3] Crystallography & NMR system:: A new software suite for macromolecular structure determination [J].

Brunger, AT ;

Adams, PD ;

Clore, GM ;

DeLano, WL ;

Gros, P ;

Grosse-Kunstleve, RW ;

Jiang, JS ;

Kuszewski, J ;

Nilges, M ;

Pannu, NS ;

Read, RJ ;

Rice, LM ;

Simonson, T ;

Warren, GL .

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 1998, 54 :905-921

[4] Disulphide-bond pattern and molecular modelling of the dimeric disintegrin EMF-10, a potent and selective integrin α5β1 antagonist from Eristocophis macmahoni venom [J].

Calvete, JJ ;

Jürgens, M ;

Marcinkiewicz, C ;

Romero, A ;

Schrader, M ;

Niewiarowski, S .

BIOCHEMICAL JOURNAL, 2000, 345 :573-581

[5] A stochastic approach to molecular replacement [J].

Glykos, NM ;

Kokkinidis, M .

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 2000, 56 :169-174

[6] Rapid automated molecular replacement by evolutionary search [J].

Kissinger, CR ;

Gehlhaar, DK ;

Fogel, DB .

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 1999, 55 :484-491

[7] Structural and functional characterization of EMF10, a heterodimeric disintegrin from Eristocophis macmahoni venom that selectively inhibits α5β1 integrin [J].

Marcinkiewicz, C ;

Calvete, JJ ;

Vijay-Kumar, S ;

Marcinkiewicz, MM ;

Raida, M ;

Schick, P ;

Lobb, PR ;

Niewiarowski, S .

BIOCHEMISTRY, 1999, 38 (40) :13302-13309

[8] EC3, a novel heterodimeric disintegrin from Echis carinatus venom, inhibits α4 and α5 integrins in an RGD-independent manner [J].

Marcinkiewicz, C ;

Calvete, JJ ;

Marcinkiewicz, MM ;

Raida, M ;

Vijay-Kumar, S ;

Huang, ZW ;

Lobb, RR ;

Niewiarowski, S .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (18) :12468-12473

[9] SOLVENT CONTENT OF PROTEIN CRYSTALS [J].

MATTHEWS, BW .

JOURNAL OF MOLECULAR BIOLOGY, 1968, 33 (02) :491-+

[10] AMORE - AN AUTOMATED PACKAGE FOR MOLECULAR REPLACEMENT [J].

NAVAZA, J .

ACTA CRYSTALLOGRAPHICA SECTION A, 1994, 50 :157-163