Unfolding and intermolecular association in globular proteins adsorbed at interfaces

被引:132
作者
Green, RJ [1 ]
Hopkinson, I [1 ]
Jones, RAL [1 ]
机构
[1] Univ Cambridge, Cavendish Lab, Cambridge CB3 0HE, England
关键词
D O I
10.1021/la981052t
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
The conformational transitions that occur on heating solutions of globular proteins, unfolding and aggregation, were compared with the analogous transitions undergone by proteins adsorbed at interfaces. Fourier transform infrared spectrometry in solution and in the attenuated total reflection geometry revealed, for the globular proteins hen egg lysozyme and bovine serum albumen, both qualitative and quantitative differences between the transitions as they occur in bulk and adsorbed at an interface. In the bulk, unfolding is a sharp transition, followed sequentially on further heating by the relatively sharp onset of the intermolecular association associated with heat set gelation. In contrast, for adsorbed proteins, we found that both processes occur simultaneously over a wide range of temperatures. Proteins were more structurally stable adsorbed at a relatively hydrophilic, solid surface than at a liquid, hydrophobic surface; in the latter case, onset temperatures for both unfolding and intermolecular association were substantially lower than for bulk solutions.
引用
收藏
页码:5102 / 5110
页数:9
相关论文
共 31 条
[1]  
ANDRADE JD, 1985, PRINCIPLES PROTEIN A, V2, P1
[2]   CONFORMATIONAL-CHANGES IN ADSORBED PROTEINS [J].
BALL, A ;
JONES, RAL .
LANGMUIR, 1995, 11 (09) :3542-3548
[3]   Effects of physicochemical factors on the secondary structure of beta-lactoglobulin [J].
Boye, JI ;
Ismail, AA ;
Alli, I .
JOURNAL OF DAIRY RESEARCH, 1996, 63 (01) :97-109
[4]  
Brash J. L., 1995, ACS SYM SER, V602, P1
[5]   Adsorption kinetics, conformation, and mobility of the growth hormone and lysozyme on solid surfaces, studied with TIRF [J].
Buijs, J ;
Hlady, V .
JOURNAL OF COLLOID AND INTERFACE SCIENCE, 1997, 190 (01) :171-181
[6]  
BYLER DM, 1989, P SOC PHOTO-OPT INS, V1145, P415
[7]   A pH dependent coil-to-sheet transition in a periodic artificial protein adsorbed at the air-water interface [J].
Chen, HJ ;
Hsu, SL ;
Tirrell, DA ;
Stidham, HD .
LANGMUIR, 1997, 13 (18) :4775-4778
[8]  
CLARK AH, 1980, INT J PEPT PROT RES, V16, P339
[9]  
CLARK AH, 1981, INT J PEPT PROT RES, V17, P380
[10]  
CLARK AH, 1981, INT J PEPT PROT RES, V17, P353