Osteogenic growth peptide promotes osteogenic differentiation of mesenchymal stem cells mediated by LncRNA AK141205-induced upregulation of CXCL13

被引：77

作者：

Li, Haiqing ^{[1
]}

Zhang, Zhonghe ^{[3
]}

Chen, Zhiqiang ^{[1
]}

Zhang, Dongdong ^{[2
]}

机构：

[1] Liaocheng Peoples Hosp, Dept Orthoped Surg, Liaocheng 252000, Peoples R China

[2] Liaocheng Peoples Hosp, Dept Ultrasound, Liaocheng 252000, Peoples R China

[3] Liaocheng Hosp Tradit Chinese Med, Dept Orthoped Surg, Liaocheng 252000, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2015年 / 466卷 / 01期

关键词：

Alkaline phosphatase (ALP) activity; AK141205; Mesenchymal stem cells; Osteogenic growth peptide; Runx2; STROMAL CELLS; OSTEOBLAST DIFFERENTIATION; CHEMOKINE; RUNX2;

D O I：

10.1016/j.bbrc.2015.08.112

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

The aim of present study was to characterize long non-coding RNA (IncRNA) AK141205 as a cellular regulator of osteogenic differentiation of mice mesenchymal stem cells (MSCs) towards osteogenic growth peptide (OGP) stimulation. Mice MSCs cells were isolated, transfected with si-AK141205, pcDNA-AK141205 or control, and stimulated with OCR The AK141205, CXC chemokine ligand-13 (CXCL13), and osteogenic differentiation-associated parameters were determined by western blotting or quantitative RT-PCR. To determine the role of AK141205/CXCL13 in SMCs osteogenic differentiation, SMCs subjected to co-transfection of pcDNA-AK141205 and si-CXCL13 or si-AK141205 and pcDNA-CXCL13, and were submitted for osteogenic differentiation-associated parameters analyses. The results showed that stimulation of SMCs with OGP induced upregulation of both AK141205 and CXCL13, and osteogenic differentiation of MSCs. Transfection of si-AK141205 partly suppressed OGP-induced formation of calcium salt nodules, alkaline phosphatase (ALP) activity and osteogenic differentiation-associated gene expression, suggesting key regulatory role of AK141205. Analysis of CXCL13 expression in SMCspcDNA-AK141205 revealed that AK141205 positively promoted CXCL13 expression via acetylation of H4 histone in the promoter region. This signal transduction was demonstrated to be essential for OGP-induced osteogenic differentiation of MSCs through osteogenic differentiation analysis in simultaneously AK141205/CXCL13 controlled SMCs. In summary, we report a completely novel role of AK141205/CXCL13 as a regulator of OGP-induced osteogenic differentiation of SMCs. Our finding provides a potential therapeutic targeting of AK141205 for enhancing disease-treatment effect of SMCs. (C) 2015 Elsevier Inc. All rights reserved.

引用

页码：82 / 88

页数：7

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