Crystallization and initial X-ray analysis of rabbit mature sterol carrier protein 2

被引：6

作者：

Choinowski, T ^{[1
]}

Dyer, JH ^{[1
]}

Maderegger, B ^{[1
]}

Winterhalter, KH ^{[1
]}

Hauser, H ^{[1
]}

Piontek, K ^{[1
]}

机构：

[1] Swiss Fed Inst Technol, Inst Biochem, CH-8092 Zurich, Switzerland

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY | 1999年 / 55卷

关键词：

D O I：

10.1107/S0907444999007106

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Sterol carrier protein 2 (SCP2) is a basic intracellular protein which facilitates the in vitro intermembrane transfer of cholesterol, phospholipids and glycolipids. SCP2 was expressed in Escherichia coli, purified to apparent electrophoretic homogeneity and crystallized. Single crystals were obtained by hanging-drop vapour diffusion using ammonium sulfate as precipitant. These crystals belong to space group P4(1)2(1)2 or its enantiomorph, with unit-cell parameters a = b = 57.5, c = 86.5 Angstrom, and have one molecule in the crystallographic asymmetric unit. Intensity data to 1.8 Angstrom resolution were collected from native SCP2 crystals using synchrotron radiation, were processed and scaled with an R-linear = 4.9%.

引用

页码：1478 / 1480

页数：3

共 22 条

[1]

BLOJ B, 1977, J BIOL CHEM, V252, P1613

[2]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[3] TREATMENT OF NEGATIVE INTENSITY OBSERVATIONS [J].

FRENCH, S ;

WILSON, K .

ACTA CRYSTALLOGRAPHICA SECTION A, 1978, 34 (JUL) :517-525

[4] Sterol carrier protein-2, a new fatty acyl coenzyme A-binding protein [J].

Frolov, A ;

Cho, TH ;

Billheimer, JT ;

Schroeder, F .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (50) :31878-31884

[5] SPARSE-MATRIX SAMPLING - A SCREENING METHOD FOR CRYSTALLIZATION OF PROTEINS [J].

JANCARIK, J ;

KIM, SH .

JOURNAL OF APPLIED CRYSTALLOGRAPHY, 1991, 24 :409-411

[6] SOLVENT CONTENT OF PROTEIN CRYSTALS [J].

MATTHEWS, BW .

JOURNAL OF MOLECULAR BIOLOGY, 1968, 33 (02) :491-+

[7]

MEYERSPAYNE SC, 1996, J NEUROCHEM, V66, P313

[8]

NOLAND BJ, 1980, J BIOL CHEM, V255, P4282

[9] THE NONSPECIFIC LIPID-TRANSFER PROTEIN (STEROL CARRIER PROTEIN 2) AND ITS RELATIONSHIP TO PEROXISOMES [J].

OSSENDORP, BC ;

WIRTZ, KWA .

BIOCHIMIE, 1993, 75 (3-4) :191-200

[10] IDENTIFICATION OF THE CDNA CLONE WHICH ENCODES THE 58-KDA PROTEIN CONTAINING THE AMINO-ACID-SEQUENCE OF RAT-LIVER NONSPECIFIC LIPID-TRANSFER PROTEIN (STEROL-CARRIER PROTEIN 2) - HOMOLOGY WITH RAT PEROXISOMAL AND MITOCHONDRIAL 3-OXOACYL-COA THIOLASES [J].

OSSENDORP, BC ;

VANHEUSDEN, GPH ;

DEBEER, ALJ ;

BOS, K ;

SCHOUTEN, GL ;

WIRTZ, KWA .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1991, 201 (01) :233-239

← 1 2 3 →