Conserved cis-elements in the Xenopus red opsin promoter necessary for cone-specific expression

被引：6

作者：

Babu, S ^{[1
]}

McIlvain, V ^{[1
]}

Whitaker, SL ^{[1
]}

Knox, BE ^{[1
]}

机构：

[1] SUNY Upstate Med Univ, Dept Biochem & Mol Biol & Ophthalmol, Syracuse, NY 13210 USA

来源：

FEBS LETTERS | 2006年 / 580卷 / 05期

关键词：

retina; transcription; gene expression; photoreceptor; promoter; transgenic;

D O I：

10.1016/j.febslet.2006.01.080

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The long-wavelength sensitive (red) opsin genes encode proteins which play a central role in daytime and color vision in vertebrates. We used transgenic Xenopus to identify 5' cis-elements in the red cone opsin promoter necessary for cone-specific expression. We found a highly conserved extended region (-725 to -173) that was required for restricting GFP transgene expression to cones. We further identified a short element (5'-CCAATTAAG-AGAT-3') highly conserved amongst tetrapods, including humans, necessary to restrict expression to cones in the retina. These results identify novel conserved elements that regulate spatial expression of tetrapod red cone opsin genes. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：1479 / 1484

页数：6

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