Influence of He-Ne laser irradiation on the excision repair of cyclobutyl pyrimidine dimers in the wheat DNA

被引：22

作者：

Han, R

Wang, XL ^{[1
]}

Yue, M

机构：

[1] NW Univ Xian, Sch Life Sci, Xian 710069, Peoples R China

[2] Shanxi Normal Univ, Sch Life Sci, Linfen 041004, Peoples R China

[3] Lanzhou Univ, Sch Life Sci, Lanzhou 730000, Peoples R China

来源：

CHINESE SCIENCE BULLETIN | 2002年 / 47卷 / 10期

基金：

中国国家自然科学基金;

关键词：

wheat; ultraviolet-B; He-Ne laser; DNA excision repair;

D O I：

10.1360/02tb9184

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Using agarose gel electrophoresis and T4-endodeoxyribonuclease-V, which is a kind of restriction endonuclease of cyclobutyl pyrimidine dimer (CPD), the impacts of He-Ne laser (5 mW (.) mm(-2)) irradiation on DNA excision repair capacity in damaged wheat cells induced by enhanced ultraviolet-B (10.08 kJ (.) m(-2 .) d(-1)) radiation were studied. The results indicated that the content of endonuclease sensitive sites (ESS) was reduced by He-Ne laser irradiation, which formed in cells irradiated by enhanced ultraviolet-B. With the irradiation of He-Ne laser, the excision of CPDs and the reduction of single strand breaks (SSB) contents which were the endonuclease sensitive sites (ESS) digested by T4-endodeoxyribonuclease-V had been stimulated in the wheat cells.

引用

页码：818 / 821

页数：4

共 19 条

[1] Ozone depletion and increased UV-B radiation:: is there a real threat to photosynthesis? [J].