MG53 Regulates Membrane Budding and Exocytosis in Muscle Cells

被引：100

作者：

Cai, Chuanxi ^{[2
]}

Masumiya, Haruko ^{[1
]}

Weisleder, Noah ^{[2
]}

Pan, Zui ^{[2
]}

Nishi, Miyuki ^{[1
,4
]}

Komazaki, Shinji ^{[5
]}

Takeshima, Hiroshi ^{[1
,4
]}

Ma, Jianjie ^{[2
,3
]}

机构：

[1] Tohoku Univ, Grad Sch Med, Dept Med Chem, Sendai, Miyagi 9808575, Japan

[2] Univ Med & Dent New Jersey, Robert Wood Johnson Med Sch, Dept Physiol & Biophys, Piscataway, NJ 08854 USA

[3] Univ Med & Dent New Jersey, Robert Wood Johnson Med Sch, Dept Med, Piscataway, NJ 08854 USA

[4] Kyoto Univ, Grad Sch Pharmaceut Sci, Dept Biol Chem, Kyoto 6068501, Japan

[5] Saitama Med Univ, Dept Anat, Saitama 3500495, Japan

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 2009年 / 284卷 / 05期

基金：

美国国家卫生研究院;

关键词：

DUCHENNE-MUSCULAR-DYSTROPHY; RING-FINGER PROTEIN; SKELETAL-MUSCLE; RYANODINE RECEPTORS; MONKEY TRIM5-ALPHA; SARCOPLASMIC-RETICULUM; TRIAD JUNCTION; CAVEOLIN-3; RESTRICTION; DOMAINS;

D O I：

10.1074/jbc.M808866200

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Membrane recycling and remodeling contribute to multiple cellular functions, including cell fusion events during myogenesis. We have identified a tripartite motif (TRIM72) family member protein named MG53 and defined its role in mediating the dynamic process of membrane fusion and exocytosis in striated muscle. MG53 is a muscle-specific protein that contains a TRIM motif at the amino terminus and a SPRY motif at the carboxyl terminus. Live cell imaging of green fluorescent protein-MG53 fusion construct in cultured myoblasts showed that although MG53 contains no trans-membrane segment it is tightly associated with intracellular vesicles and sarcolemmal membrane. RNA interference-mediated knockdown of MG53 expression impeded myoblast differentiation, whereas overexpression of MG53 enhanced vesicle trafficking to and budding from sarcolemmal membrane. Co-expression studies indicated that MG53 activity is regulated by a functional interaction with caveolin-3. Our data reveal a new function for TRIM family proteins in regulating membrane trafficking and fusion in striated muscles.

引用

页码：3314 / 3322

页数：9

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