The Membrane Protein ATPase Class I Type 813 Member 1 Signals Through Protein Kinase C Zeta To Activate the Farnesoid X Receptor

被引：72

作者：

Frankenberg, Tamara ^{[1
]}

Miloh, Tamir ^{[1
]}

Chen, Frank Y. ^{[2
,3
]}

Ananthanarayanan, Meena ^{[1
]}

Sun, An-Qiang ^{[1
]}

Balasubramaniyan, Natarajan ^{[1
]}

Arias, Irwin ^{[4
]}

Setchell, Kenneth D. R. ^{[5
,6
]}

Suchy, Frederick J. ^{[1
]}

Shneider, Benjamin L. ^{[2
,3
]}

机构：

[1] Mt Sinai Sch Med, Dept Pediat, New York, NY USA

[2] Univ Pittsburgh, Med Ctr, Childrens Hosp Pittsburgh, Div Pediat Gastroenterol Hepatol & Nutr, Pittsburgh, PA 15213 USA

[3] Univ Pittsburgh, Sch Med, Dept Pediat, Pittsburgh, PA 15213 USA

[4] NICHHD, Cell Biol & Metab Branch, NIH, Bethesda, MD 20892 USA

[5] Childrens Hosp, Med Ctr, Dept Pathol, Cincinnati, OH 45229 USA

[6] Univ Cincinnati, Coll Med, Dept Pediat, Cincinnati, OH USA

来源：

HEPATOLOGY | 2008年 / 48卷 / 06期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1002/hep.22431

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

Prior loss-of-function analyses revealed that ATPase class I type 8B member 1 [familial intrahepatic cholestasis 1 (FICI)] posttranslationally activated the farnesoid X receptor (FXR). Mechanisms underlying this regulation were examined by gain-of-function studies in UPS cells, which lack endogenous FICI expression. FXR function was assayed in response to wild-type and mutated FICI expression constructs with a human bile salt export pump (BSEP) promoter and a variety of cellular localization techniques. FICI overexpression led to enhanced phosphorylation and nuclear localization of FXR that was associated with FXR-dependent activation of the BSEP promoter. The FICI effect was lost after mutation of the FXR response element in the BSEP promoter. Despite similar levels of FICI protein expression, Byler disease FICI mutants did not activate BSEP, whereas benign recurrent intrahepatic cholestasis mutants partially activated BSEP. The FICI effect was dependent on the presence of the FXR ligand, chenodeoxycholic acid. The effect of FICI on FXR phosphorylation and nuclear localization and its effects on BSEP promoter activity could be blocked with protein kinase C zeta (PKC zeta) inhibitors (pseudosubstrate or small interfering RNA silencing). Recombinant PKC zeta directly phosphorylated immunoprecipitated FXR. The mutation of threonine 442 of FXR to alanine yielded a dominant negative protein, whereas the phosphomimetic conversion to glutamate resulted in FXR with enhanced activity and nuclear localization. Inhibition of PKC zeta in Caco-2 cells resulted in activation of the human apical sodium-dependent bile acid transporter promoter. Conclusion: These results demonstrate that FICI signals to FXR via PKC. FICI-related liver disease is likely related to downstream effects of FXR on bile acid homeostasis. Benign recurrent intrahepatic cholestasis emanates from a partially functional FICI protein. Phosphorylation of FXR is an important mechanism for regulating its activity. (HEPATOLOGY 2008;48:1896-1905.)

引用

页码：1896 / 1905

页数：10

共 54 条

[1] Reduced hepatic expression of farnesoid X receptor in hereditary cholestasis associated to mutation in ATP8B1 [J].