Phylogeny of the filamentous bacterium Eikelboom Type 1851, and design and application of a 16S rRNA targeted oligonucleotide probe for its fluorescence in situ identification in activated sludge

被引：43

作者：

Beer, M

Seviour, EM

Kong, Y

Cunningham, M

Blackall, LL

Seviour, RJ ^{[1
]}

机构：

[1] La Trobe Univ, Biotechnol Res Ctr, Bendigo, Vic 3552, Australia

[2] Univ Queensland, Dept Microbiol & Parasitol, Brisbane, Qld 4072, Australia

来源：

FEMS MICROBIOLOGY LETTERS | 2002年 / 207卷 / 02期

关键词：

filamentous bacterium; activated sludge; bulking; Type; 1851; 'Chloroflexi'; green non-sulfur bacterium; fluorescence in situ hybridization;

D O I：

10.1016/S0378-1097(01)00561-4

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Micromanipulation US used to obtain an isolate (BEN 52) of Eikeiboom Type 1851 from a bulking activated sludge plant. Its 16S rDNA sequence reveals its closest relative is 'Roseiflexus castenholzii'. a member of the phylum 'Chloroflexi', class 'Chloroflexi'. Previously called the green non-sulfur bacteria. The 16S rRNA targeted oligonucleotide probe designed for fluorescence in Situ hybridisation against this sequence Successfully identified filamentous bacteria with the morphological features of Type 1851 in activated sludge samples from plants in several countries and different operational Configurations. (C) 21002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：179 / 183

页数：5

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