Nucleoside triphosphate diphosphohydrolase-2 is the ecto-ATPase of type I cells in taste buds

被引:212
作者
Bartel, Dianna L.
Sullivan, Susan L.
Lavoie, Elise G.
Sevigny, Jean
Finger, Thomas E.
机构
[1] UCDHSC, Dept Cell & Dev Biol, Rocky Mt Taste & Smell Ctr, Aurora, CO 80045 USA
[2] Natl Inst Deafness & Other Commun Disorders, NIH, Rockville, MD 20850 USA
[3] Univ Laval, Ctr Rech Rhumatol & Immunol, Quebec City, PQ G1V 4G2, Canada
关键词
gustatory; ecto-ATPase; NTPDase2; ear; ATP signaling; mouse; taste bud;
D O I
10.1002/cne.20954
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
The presence of one or more calcium-dependent ecto-ATPases (enzymes that hydrolyze extracellular 5'-triphosphates) in mammalian taste buds was first shown histochemically. Recent studies have established that dominant ecto-ATPases consist of enzymes now called nucleoside triphosphate diphosphohydrolases (NTPDases). Massively parallel signature sequencing (MPSS) from murine taste epithelium provided molecular evidence suggesting that NTPDase2 is the most likely member present in mouse taste papillae. Immunocytochemical and enzyme histochemical staining verified the presence of NTPDase2 associated with plasma membranes in a large number of cells within all mouse taste buds. To determine which of the three taste cell types expresses this enzyme, double-label assays were performed with antisera directed against the glial glutamate/aspartate transporter (GLAST), the transduction pathway proteins phospholipase C beta 2 (PLC beta 2) or the G-protein subunit a-gustducin, and serotonin (5HT) as markers of type I, II, and III taste cells, respectively. Analysis of the double-labeled sections indicates that NTPDase2 immunoreactivity is found on cell processes that often envelop other taste cells, reminiscent of type I cells. In agreement with this observation, NTPDase2 was located to the same membrane as GLAST, indicating that this enzyme is present in type I cells. The presence of ecto-ATPase in taste buds likely reflects the importance of ATP as an intercellular signaling molecule in this system.
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页码:1 / 12
页数:12
相关论文
共 56 条
[1]   Purinergic signalling: Pathophysiological roles [J].
Abbracchio, MP ;
Burnstock, G .
JAPANESE JOURNAL OF PHARMACOLOGY, 1998, 78 (02) :113-145
[2]   A novel family of mammalian taste receptors [J].
Adler, E ;
Hoon, MA ;
Mueller, KL ;
Chandrashekar, J ;
Ryba, NJP ;
Zuker, CS .
CELL, 2000, 100 (06) :693-702
[3]   FINE-STRUCTURAL LOCALIZATION OF ADENOSINE-TRIPHOSPHATASE ACTIVITY ON TASTE BUD IN FUNGIFORM PAPILLAE OF RAT [J].
AKISAKA, T ;
ODA, M .
ARCHIVUM HISTOLOGICUM JAPONICUM, 1977, 40 (01) :63-72
[4]   ECTO-CALCIUM-DEPENDENT ATPASE ACTIVITY OF MAMMALIAN TASTE BUD CELLS [J].
BARRY, MA .
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1992, 40 (12) :1919-1928
[5]   Calcium signaling mediated by P2Y receptors in mouse taste cells [J].
Baryshnikov, SG ;
Rogachevskaja, OA ;
Kolesnikov, SS .
JOURNAL OF NEUROPHYSIOLOGY, 2003, 90 (05) :3283-3294
[6]   Cloning and characterization of mouse nucleoside triphosphate diphosphohydrolase-8 [J].
Bigonnesse, F ;
Lévesque, SA ;
Kukulski, F ;
Lecka, J ;
Robson, SC ;
Fernandes, MJG ;
Sévigny, J .
BIOCHEMISTRY, 2004, 43 (18) :5511-5519
[7]   Localization of ATP-gated P2X2 and P2X3 receptor immunoreactive nerves in rat taste buds [J].
Bo, XN ;
Alavi, A ;
Xiang, ZH ;
Oglesby, I ;
Ford, A ;
Burnstock, G .
NEUROREPORT, 1999, 10 (05) :1107-1111
[8]  
Boughter JD, 1997, J NEUROSCI, V17, P2852
[9]   Association of the Ecto-ATPase NTPDase2 with glial cells of the peripheral nervous system [J].
Braun, N ;
Sévigny, J ;
Robson, SC ;
Hammer, K ;
Hanani, M ;
Zimmermann, H .
GLIA, 2004, 45 (02) :124-132
[10]   Expression of the ecto-ATPase NTPDase2 in the germinal zones of the developing and adult rat brain [J].
Braun, N ;
Sévigny, J ;
Mishra, SK ;
Robson, SC ;
Barth, SW ;
Gerstberger, R ;
Hammer, K ;
Zimmermann, H .
EUROPEAN JOURNAL OF NEUROSCIENCE, 2003, 17 (07) :1355-1364