Micropatterning gradients and controlling surface densities of photoactivatable biomolecules on self-assembled monolayers of oligo(ethylene glycol) alkanethiolates

被引:129
作者
Herbert, CB
McLernon, TL
Hypolite, CL
Adams, DN
Pikus, L
Huang, CC
Fields, GB
Letourneau, PC
Distefano, MD
Hu, WS
机构
[1] UNIV MINNESOTA,DEPT CHEM ENGN & MAT SCI,MINNEAPOLIS,MN 55455
[2] UNIV MINNESOTA,DEPT CHEM,MINNEAPOLIS,MN 55455
[3] UNIV MINNESOTA,DEPT PHYS,MINNEAPOLIS,MN 55455
[4] UNIV MINNESOTA,LAB MED & PATHOL,MINNEAPOLIS,MN 55455
[5] UNIV MINNESOTA,DEPT CELL BIOL & NEUROANAT,MINNEAPOLIS,MN 55455
来源
CHEMISTRY & BIOLOGY | 1997年 / 4卷 / 10期
基金
美国国家科学基金会; 美国国家卫生研究院;
关键词
benzophenone; cultured cells; micropattern; photochemistry; self-assembled monolayer;
D O I
10.1016/S1074-5521(97)90311-2
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Background: Bioactive molecules that are covalently immobilized in patterns on surfaces have previously been used to control or study cell behavior such as adhesion, spreading, movement or differentiation. Photoimmobilization techniques can be used, however, to control not only the spatial pattern of molecular immobilization, termed the micropattern, but also the surface density of the molecules - a characteristic that has not been previously exploited. Results: Oligopeptides containing the bioactive Arg-Gly-Asp cell-adhesion sequence were immobilized upon self-assembled monolayers of an oligo(ethylene glycol) alkanethiolate in patterns that were Visualized and quantified by autoradiography. The amount and pattern of immobilized peptide were controlled by manipulating the exposure of the sample to a UV lamp or a laser beam. Patterns of peptides, including a density gradient, were used to control the location and number of adherent cells and also the cell shape. Conclusions: A photoimmobilization technique for decorating surfaces with micropatterns that consist of variable densities of bioactive molecules is described. The efficacy of the patterns for controlling cell adhesion and shape has been demonstrated. This technique is useful for the study of cell behavior on micropatterns.
引用
收藏
页码:731 / 737
页数:7
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