Colorimetric method for a rapid detection of oxygenated aromatic biotransformation products

被引：28

作者：

Quintana, MG ^{[1
]}

Didion, C ^{[1
]}

Dalton, H ^{[1
]}

机构：

[1] UNIV WARWICK,DEPT BIOL SCI,COVENTRY CV4 7AL,W MIDLANDS,ENGLAND

来源：

BIOTECHNOLOGY TECHNIQUES | 1997年 / 11卷 / 08期

关键词：

D O I：

10.1023/A:1018499024466

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The quantitative production of the oxygenated products from the biotransformation of aromatic substrates can be detected using a very simple and rapid colorimetric test. The method is based on Gibbs' reagent (2,6-dichloroquinone-4-chloroimide) and has been developed for routine spectrophotometric or microtitre plate assay allowing the detection of products with a sensitivity as low as 5 mu M.

引用

页码：585 / 587

页数：3

共 18 条

[1]

BADAIRE B, 1990, B SOC PHARM BORDEAUX, V129, P137

[2] A BIOTECH ROUTE TO POLYPHENYLENE [J].

BALLARD, DGH ;

COURTIS, A ;

SHIRLEY, IM ;

TAYLOR, SC .

JOURNAL OF THE CHEMICAL SOCIETY-CHEMICAL COMMUNICATIONS, 1983, (17) :954-955

[3] ACID-CATALYZED AROMATIZATION OF BENZENE CIS-1,2-DIHYDRODIOLS - A CARBOCATION TRANSITION-STATE POORLY STABILIZED BY RESONANCE [J].

BOYD, DR ;

BLACKER, J ;

BYRNE, B ;

DALTON, H ;

HAND, MV ;

KELLY, SC ;

OFERRALL, RAM ;

RAO, SN ;

SHARMA, ND ;

SHELDRAKE, GN .

JOURNAL OF THE CHEMICAL SOCIETY-CHEMICAL COMMUNICATIONS, 1994, (03) :313-314

[4] STRUCTURES AND STEREOCHEMICAL ASSIGNMENTS OF SOME NOVEL CHIRAL SYNTHONS DERIVED FROM THE BIOTRANSFORMATION OF 2,3-DIHYDROBENZOFURAN AND BENZOFURAN BY PSEUDOMONAS-PUTIDA [J].

BOYD, DR ;

SHARMA, ND ;

BOYLE, R ;

MALONE, JF ;

CHIMA, J ;

DALTON, H .

TETRAHEDRON-ASYMMETRY, 1993, 4 (06) :1307-1324

[5] STEREODIRECTING SUBSTITUENT EFFECTS DURING ENZYME-CATALYZED SYNTHESIS OF CIS-DIHYDRODIOL METABOLITES OF 1,4-DISUBSTITUTED BENZENE SUBSTRATES [J].

BOYD, DR ;

SHARMA, ND ;

HAND, MV ;

GROOCOCK, MR ;

KERLEY, NA ;

DALTON, H ;

CHIMA, J ;

SHELDRAKE, GN .

JOURNAL OF THE CHEMICAL SOCIETY-CHEMICAL COMMUNICATIONS, 1993, (11) :974-976

[6] TOLUENE CIS-GLYCOL SYNTHESIS BY PSEUDOMONAS-PUTIDA - KINETIC DATA FOR REACTOR EVALUATION [J].

BRAZIER, AJ ;

LILLY, MD ;

HERBERT, AB .

ENZYME AND MICROBIAL TECHNOLOGY, 1990, 12 (02) :90-94

[7] NONSPECIFICITY OF GIBBS REACTION [J].

DACRE, JC .

ANALYTICAL CHEMISTRY, 1971, 43 (04) :589-&

[8]

Gibbs HD, 1927, J BIOL CHEM, V72, P649

[9] OXIDATIVE DEGRADATION OF AROMATIC HYDROCARBONS BY MICROORGANISMS .2. METABOLISM OF HALOGENATED AROMATIC HYDROCARBONS [J].

GIBSON, DT ;

KOCH, JR ;

SCHULD, CL ;

KALLIO, RE .

BIOCHEMISTRY, 1968, 7 (11) :3795-&

[10] TRANSITION-METAL-CATALYZED POLYMERIZATION OF HETEROATOM-FUNCTIONALIZED CYCLOHEXADIENES - STEREOREGULAR PRECURSORS TO POLY(PARA-PHENYLENE) [J].

GIN, DL ;

CONTICELLO, VP ;

GRUBBS, RH .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1992, 114 (08) :3167-3169

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