From gene switches to mammalian designer cells: present and future prospects

被引：108

作者：

Auslaender, Simon ^{[1
]}

Fussenegger, Martin ^{[1
,2
]}

机构：

[1] Swiss Fed Inst Technol, Dept Biosyst Sci & Engn, CH-4058 Basel, Switzerland

[2] Univ Basel, Fac Sci, CH-4058 Basel, Switzerland

来源：

TRENDS IN BIOTECHNOLOGY | 2013年 / 31卷 / 03期

关键词：

synthetic biology; biocomputer; mammalian gene regulation; cell implants; gene circuits; CONTROLLING TRANSGENE EXPRESSION; TRANSCRIPTION CONTROL; PROTEIN INTERACTIONS; RNA INTERFERENCE; LIVING CELLS; SYSTEM; INDUCTION; CIRCUIT; DEGRADATION; HOMEOSTASIS;

D O I：

10.1016/j.tibtech.2012.11.006

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 090105 [作物生产系统与生态工程];

摘要：

Nature has evolved a treasury of biological molecules that are logically connected to networks, enabling cells to maintain their functional integrity. Similar to electronic circuits, cells operate as information-processing systems that dynamically integrate and respond to distinct input signals. Synthetic biology aims to standardize and expand the natural toolbox of biological building blocks to engineer novel synthetic networks in living systems. Mammalian cells harboring integrated designer circuits could work as living biocomputers that execute predictable metabolic and therapeutic functions. This review presents design principles of mammalian gene circuits, highlights recent developments, and discusses future challenges and prospects.

引用

页码：155 / 168

页数：14

共 95 条

[1]

Artificial control of gene expression in mammalian cells by modulating RNA interference through aptamer-small molecule interaction [J].

An, CI ;

Trinh, VB ;

Yokobayashi, Y .

RNA, 2006, 12 (05) :710-716

[2]

Rational design of a small molecule-responsive intramer controlling transgene expression in mammalian cells [J].

Auslaender, David ;

Wieland, Markus ;

Auslaender, Simon ;

Tigges, Marcel ;

Fussenegger, Martin .

NUCLEIC ACIDS RESEARCH, 2011, 39 (22) :e155

[3]

Programmable single-cell mammalian biocomputers [J].

Auslaender, Simon ;

Auslaender, David ;

Mueller, Marius ;

Wieland, Markus ;

Fussenegger, Martin .

NATURE, 2012, 487 (7405) :123-+

[4]

Smart medication through combination of synthetic biology and cell microencapsulation [J].

Auslaender, Simon ;

Wieland, Markus ;

Fussenegger, Martin .

METABOLIC ENGINEERING, 2012, 14 (03) :252-260

[5]

A ligand-dependent hammerhead ribozyme switch for controlling mammalian gene expression [J].

Auslaender, Simon ;

Ketzer, Patrick ;

Hartig, Joerg S. .

MOLECULAR BIOSYSTEMS, 2010, 6 (05) :807-814

[6]

Synthetic two-way communication between mammalian cells [J].

Bacchus, William ;

Lang, Moritz ;

El-Baba, Marie Daoud ;

Weber, Wilfried ;

Stelling, Joerg ;

Fussenegger, Martin .

NATURE BIOTECHNOLOGY, 2012, 30 (10) :991-+

[7]

Human zinc fingers as building blocks in the construction of artificial transcription factors [J].

Bae, KH ;

Do Kwon, Y ;

Shin, HC ;

Hwang, MS ;

Ryu, EH ;

Park, KS ;

Yang, HY ;

Lee, D ;

Lee, Y ;

Park, J ;

Kwon, HS ;

Kim, HW ;

Yeh, BI ;

Lee, HW ;

Sohn, SH ;

Yoon, J ;

Seol, W ;

Kim, JS .

NATURE BIOTECHNOLOGY, 2003, 21 (03) :275-280

[8]

A rapid, reversible, and tunable method to regulate protein function in living cells using synthetic small molecules [J].

Banaszynski, Laura A. ;

Chen, Lin-chun ;

Maynard-Smith, Lystranne A. ;

Ooi, A. G. Lisa ;

Wandless, Thomas J. .

CELL, 2006, 126 (05) :995-1004

[9]

Chemically regulated zinc finger transcription factors [J].

Beerli, RR ;

Schopfer, U ;

Dreier, B ;

Barbas, CF .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (42) :32617-32627

[10]

Design of small molecule-responsive microRNAs based on structural requirements for Drosha processing [J].

Beisel, Chase L. ;

Chen, Yvonne Y. ;

Culler, Stephanie J. ;

Hoff, Kevin G. ;

Smolke, Christina D. .

NUCLEIC ACIDS RESEARCH, 2011, 39 (07) :2981-2994

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