Tyrosine phosphorylation induced by C4 peptide constructs from HIV Gp120

被引：6

作者：

Liu, MF

Zeng, J

Robey, FA ^{[1
]}

机构：

[1] NIDR, Oral & Pharyngeal Canc Branch, Bethesda, MD 20892 USA

[2] NCI, Biochem Lab, NIH, Bethesda, MD 20892 USA

来源：

PEPTIDES | 1999年 / 20卷 / 02期

基金：

美国国家卫生研究院;

关键词：

peptomer; gp120; synthetic peptide; signal transduction; HUT78; T cells;

D O I：

10.1016/S0196-9781(98)00158-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Treatment of HUT78 cells with CD4-binding peptide constructs derived from the C4 domain of HIV-1 gp120 results in autophosphorylation of a src-related kinase, p56(lck). This leads to p56(lck) activation and the subsequent phosphorylation of tyrosine residues in several intracellular proteins. The phosphorylation is specific to the C4 peptides as no new phosphorylation occurs when the cells are treated with control peptides or polymers. The induction of tyrosine phosphorylation by the C4 peptide constructs depends on the capability of the peptide to assume a helical conformation because similar peptide constructs that were not able to form helices did not induce cellular tyrosine phosphorylation. (C) 1999 Elsevier Science Inc. All rights reserved.

引用

页码：185 / 191

页数：7

共 43 条

[1]

AGARWAL A, 1993, J BIOL CHEM, V268, P15900

[2] A HUMAN RECOMBINANT FAB IDENTIFIES A HUMAN-IMMUNODEFICIENCY-VIRUS TYPE 1-INDUCED CONFORMATIONAL CHANGE IN CELL SURFACE-EXPRESSED CD4 [J].