Antimicrobial Photodynamic Therapy in the Non-Surgical Treatment of Aggressive Periodontitis: Cytokine Profile in Gingival Crevicular Fluid, Preliminary Results

被引:55
作者
de Oliveira, Rafael Ramos [1 ]
Schwartz-Filho, Humberto Oswaldo [1 ]
Novaes, Arthur Belem, Jr. [1 ]
Garlet, Gustavo Pompermaier [2 ]
de Souza, Raphael Freitas [3 ]
Taba, Mario, Jr. [1 ]
de Souza, Sergio Luis Scombatti [1 ]
Ribeiro, Francisco Jeter [4 ]
机构
[1] Univ Sao Paulo, Sch Dent Ribeirao Preto, Dept Bucco Maxillofacial Surg & Traumatol & Perio, BR-14040904 Ribeirao Preto, SP, Brazil
[2] Univ Sao Paulo, Sch Dent Bauru, Dept Biol Sci, Bauru, SP, Brazil
[3] Univ Sao Paulo, Sch Dent Ribeirao Preto, Dept Dent Mat & Prostheses, BR-14040904 Ribeirao Preto, SP, Brazil
[4] Univ Fed Rio de Janeiro, Sch Dent, Dept Prosthodont, Rio De Janeiro, Brazil
关键词
Cytokines; periodontal diseases/therapy; photochemotherapy; photosensitizing agents; TUMOR-NECROSIS-FACTOR; ACTINOBACILLUS-ACTINOMYCETEMCOMITANS; PORPHYROMONAS-GINGIVALIS; LASER IRRADIATION; VIRULENCE FACTORS; ORAL BACTERIA; DISEASE; BONE; INTERLEUKIN-1; PROGRESSION;
D O I
10.1902/jop.2009.070465
中图分类号
R78 [口腔科学];
学科分类号
100302 [口腔临床医学];
摘要
Background: Aggressive periodontitis is a specific form of periodontal disease that is characterized by rapid attachment loss and bone destruction. Cytokine profiles are of considerable value when studying disease course during treatment. The aim of this trial was to investigate cytokine levels in the gingival crevicular fluid (GCF) of patients with aggressive periodontitis, after treatment with photodynamic therapy (PDT) or scaling and root planing (SRP), in a split-mouth design on -7, 0, +1, +7, +30, and +90 days. Methods: Ten patients were randomly treated with PDT using a laser source associated with a photosensitizer or SRP with hand instruments. GCF samples were collected, and the concentrations of tumor necrosis factor-alpha (TNF-alpha) and receptor activator of nuclear factor-kappa B ligand (RANKL) were determined by enzyme-linked immunosorbent assays. The data were analyzed using generalized estimating equations to test the associations among treatments, evaluated parameters, and experimental times (alpha = 0.05). Results: Non-surgical periodontal treatment with PDT or SRP led to statistically significant reductions in TNF-alpha level 30 days following treatment. There were similar levels of TNF-alpha and RANKL at the different time points in both groups, with no statistically significant differences. Conclusion: SRP and PDT had similar effects on crevicular TNF-alpha and RANKL levels in patients with aggressive periodontitis. J Periodontol 2009;80:98-105.
引用
收藏
页码:98 / 105
页数:8
相关论文
共 55 条
[1]
Armitage GC, 2003, J PERIODONTOL, V74, P1237, DOI 10.1902/jop.2003.74.8.1237
[2]
4-YEAR OBSERVATIONS OF BASIC PERIODONTAL THERAPY [J].
BADERSTEN, A ;
NIVEUS, R ;
EGELBERG, J .
JOURNAL OF CLINICAL PERIODONTOLOGY, 1987, 14 (08) :438-444
[3]
Juxtacrine cell signaling molecules [J].
Bosenberg, Marcus W. ;
Massague, Joan .
CURRENT OPINION IN CELL BIOLOGY, 1993, 5 (05) :832-838
[4]
Scaling and root planing, systemic metronidazole and professional plaque removal in the treatment of chronic periodontitis in a Brazilian population - I. Clinical results [J].
Carvalho, LH ;
D'Avila, GB ;
Leao, A ;
Haffajee, AD ;
Socransky, SS ;
Feres, M .
JOURNAL OF CLINICAL PERIODONTOLOGY, 2004, 31 (12) :1070-1076
[5]
Antimicrobial photodynamic therapy in the non-surgical treatment of aggressive periodontits: A preliminary randomized controlled clinical study [J].
de Oliveira, Rafael R. ;
Schwartz-Filho, Humberto O. ;
Novaes, Arthur B., Jr. ;
Taba, Mario, Jr. .
JOURNAL OF PERIODONTOLOGY, 2007, 78 (06) :965-973
[6]
Inflammation and tissue loss caused by periodontal pathogens is reduced by interleukin-1 antagonists [J].
Delima, AJ ;
Karatzas, S ;
Amar, S ;
Graves, DT .
JOURNAL OF INFECTIOUS DISEASES, 2002, 186 (04) :511-516
[7]
The acute inflammatory response and the role of phagocytic cells in periodontal health and disease [J].
Dennison, DK ;
VanDyke, TE .
PERIODONTOLOGY 2000, 1997, 14 :54-78
[8]
Statistical issues in periodontal research [J].
DeRouen, TA ;
Hujoel, PP ;
Mancl, LA .
JOURNAL OF DENTAL RESEARCH, 1995, 74 (11) :1731-1737
[9]
Bactericidal effect of 0.95-mW helium-neon and 5-mW indium-gallium-aluminum-phosphate laser irradiation at exposure times of 30, 60, and 120 seconds on photosensitized Staphylococcus aureus and Pseudomonas aeruginosa in vitro [J].
DeSimone, NA ;
Christiansen, C ;
Dore, D .
PHYSICAL THERAPY, 1999, 79 (09) :839-846
[10]
PHOTODYNAMIC THERAPY [J].
DOUGHERTY, TJ ;
MARCUS, SL .
EUROPEAN JOURNAL OF CANCER, 1992, 28A (10) :1734-1742