Subpopulations of GABAergic and non-GABAergic rat dorsal horn neurons express Ca2+-permeable AMPA receptors

被引:71
作者
Albuquerque, C
Lee, CJ
Jackson, AC
MacDermott, AB
机构
[1] Columbia Univ, Dept Physiol & Cellular Biophys, New York, NY 10032 USA
[2] Columbia Univ, Ctr Neurobiol & Behav, New York, NY 10032 USA
关键词
calbindin; calretinin; cobalt; NK1; receptor; spinal cord;
D O I
10.1046/j.1460-9568.1999.00691.x
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Subpopulations of alpha-amino-3-hydroxy-5-methylisoxazole-4-propionic acid (AMPA) receptors that are either permeable or impermeable to Ca2+ are expressed on dorsal horn neurons in culture. While both mediate synaptic transmission, the Ca2+-permeable AMPA receptors provide a Ca2+ signal that may result in a transient change in synaptic strength [Gu, J.G., Albuquerque, C., Lee, C.J. & MacDermott, A.B. (1996) Nature, 381, 793]. To appreciate the relevance of these receptors to dorsal horn physiology, we have investigated whether they show selective expression in identified subpopulations of dorsal horn neurons. Expression of Ca2+-permeable AMPA receptors was assayed using the kainate-induced cobalt loading technique first developed by Pruss et al. [Pruss, R.M., Akeson, R.L., Racke, M.M. & Wilburn, J.L. (1991) Neuron, 7, 509]. Subpopulations of dorsal horn neurons were identified using immunocytochemistry for gamma-aminobutyric acid (GABA), glycine, substance P receptor (NK1 receptor) and the Ca2+-binding proteins, calretinin and calbindin D28K. We demonstrate that, in dorsal horn neurons in culture, kainate-induced cobalt uptake is selectively mediated by Ca2+-permeable AMPA receptors, and that a majority of GABA and NK1 receptor-expressing neurons express Ca2+-permeable AMPA receptors. GABAergic dorsal horn neurons are important in local inhibition as well as in the regulation of transmitter release from primary afferent terminals. NK1 receptor-expressing dorsal horn neurons include many of the projection neurons in the nociceptive spino-thalamic pathway. Thus, we have identified two populations of dorsal horn neurons representing important components of dorsal horn function that express Ca2+-permeable AMPA receptors. Furthermore, we show that several subpopulations of putative excitatory interneurons defined by calretinin and calbindin expression do not express Ca2+-permeable AMPA receptors.
引用
收藏
页码:2758 / 2766
页数:9
相关论文
共 45 条
[1]   Ataxia and altered dendritic calcium signaling in mice carrying a targeted null mutation of the calbindin D28k gene [J].
Airaksinen, MS ;
Eilers, J ;
Garaschuk, O ;
Thoenen, H ;
Konnerth, A ;
Meyer, M .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (04) :1488-1493
[2]  
Albuquerque C., 1998, Society for Neuroscience Abstracts, V24, P92
[3]  
ALBUQUERQUE C, 1995, SOC NEUR ABSTR, V22
[4]   Developmental expression and self-regulation of Ca2+ entry via AMPA/KA receptors in the embryonic chick retina [J].
Allcorn, S ;
Catsicas, M ;
Mobbs, P .
EUROPEAN JOURNAL OF NEUROSCIENCE, 1996, 8 (12) :2499-2510
[5]   A SINGLE AMINO-ACID DETERMINES THE SUBUNIT-SPECIFIC SPIDER TOXIN BLOCK OF ALPHA-AMINO-3-HYDROXY-5-METHYLISOXAZOLE-4-PROPIONATE KAINATE RECEPTOR CHANNELS [J].
BLASCHKE, M ;
KELLER, BU ;
RIVOSECCHI, R ;
HOLLMANN, M ;
HEINEMANN, S ;
KONNERTH, A .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (14) :6528-6532
[6]   A SYNAPTIC MODEL OF MEMORY - LONG-TERM POTENTIATION IN THE HIPPOCAMPUS [J].
BLISS, TVP ;
COLLINGRIDGE, GL .
NATURE, 1993, 361 (6407) :31-39
[7]   DIVALENT ION PERMEABILITY OF AMPA RECEPTOR CHANNELS IS DOMINATED BY THE EDITED FORM OF A SINGLE SUBUNIT [J].
BURNASHEV, N ;
MONYER, H ;
SEEBURG, PH ;
SAKMANN, B .
NEURON, 1992, 8 (01) :189-198
[8]   MONOCLONAL-ANTIBODIES DIRECTED AGAINST THE CALCIUM-BINDING PROTEIN CALBINDIN-D-28K [J].
CELIO, MR ;
BAIER, W ;
SCHARER, L ;
GREGERSEN, HJ ;
DEVIRAGH, PA ;
NORMAN, AW .
CELL CALCIUM, 1990, 11 (09) :599-602
[9]   IMPROVED METHODS FOR COBALT FILLING AND SILVER INTENSIFICATION OF INSECT MOTOR NEURONS [J].
DAVIS, NT .
STAIN TECHNOLOGY, 1982, 57 (04) :239-244
[10]  
Engelman H. S., 1997, Society for Neuroscience Abstracts, V23, P1754