The importin/karyopherin Kap114 mediates the nuclear import of TATA-binding protein

被引：34

作者：

Morehouse, H

Buratowski, RM

Silver, PA

Buratowski, S ^{[1
]}

机构：

[1] Harvard Univ, Sch Med, Dept Biol Chem & Mol Pharmacol, Boston, MA 02115 USA

[2] Dana Farber Canc Inst, Boston, MA 02115 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1999年 / 96卷 / 22期

关键词：

D O I：

10.1073/pnas.96.22.12542

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Two high copy suppressors of temperature-sensitive TATA-binding protein (TBP) mutants were isolated. One suppressor was TIF51A, which encodes eukaryotic translation initiation factor 5A. The other high copy suppressor. YGL241W, also known as KAP114, is one of 14 importin/karyopherin proteins in yeast. These proteins mediate the transport of specific macromolecules into and out of the nucleus. Cells lacking Kap114 partially mislocalize TBP to the cytoplasm. Kap114 binds TBP in vitro, and binding is disrupted in the presence of GTP gamma S. Therefore, Kap114 is an importer of TBP into the nucleus, but alternative impart pathways must also exist.

引用

页码：12542 / 12547

页数：6

共 40 条

[1] Kap104p: A karyopherin involved in the nuclear transport of messenger RNA binding proteins [J].