Dopamine D4 receptors are heterogeneously distributed in the striosomes/matrix compartments of the striatum

被引:91
作者
Rivera, A
Cuéllar, B
Girón, FJ
Grandy, DK
de la Calle, A
Moratalla, R
机构
[1] CSIC, Consejo Super Invest Cientif, Inst Cajal, Madrid 28002, Spain
[2] Univ Malaga, Sch Sci, Dept Cell Biol, E-29071 Malaga, Spain
[3] Univ Malaga, Sch Sci, Dept Stat, E-29071 Malaga, Spain
[4] Oregon Hlth & Sci Univ, Dept Physiol & Pharmacol, Portland, OR 97201 USA
关键词
D-4 dopamine receptors; electron microscopy; immunocytochemistry; nucleus accumbens; striatum; striosome;
D O I
10.1046/j.0022-3042.2001.00702.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Two important aspects of striatal function, exploratory behaviour and motor co-ordination, require the integrity of the dopamine D-4 receptor subtype. These receptors are also implicated in the pathophysiology of certain neuropsychiatric disorders. However, the distribution of D-4 receptors in the striatum has not yet been described and this situation impairs our understanding of the anatomical substrate in which D-4 receptors function. We developed a D-4 receptor-specific antibody that has permitted us to investigate the regional and cellular localization of the receptor in the neostriatum of the rat, mouse, cat and monkey. The subcellular distribution and the synaptic organization of this receptor were also determined in the rat striatum. We found moderate levels of D-4 receptor expression in the caudoputamen and lower levels in the nucleus accumbens. These receptors were expressed in cell bodies and in the neuropil and were heterogeneously distributed among different striatal compartments, being more abundant in striosomes than in the matrix. At the subcellular level, the receptor immunoreactivity was mainly localized to dendritic shafts and spines. The prominent immunoreactivity observed in the striosomes indicates that integrative processes involved in D-4-mediated limbic behaviours occurs through the striosomes rather than accumbens, whereas the motor behaviour is based in the striatal matrix.
引用
收藏
页码:219 / 229
页数:11
相关论文
共 44 条
[1]  
[Anonymous], MOL CELLULAR MECH NE
[2]   Cellular distribution of the rat D-4 dopamine receptor protein in the CNS using anti-receptor antisera [J].
Ariano, MA ;
Wang, J ;
Noblett, KL ;
Larson, ER ;
Sibley, DR .
BRAIN RESEARCH, 1997, 752 (1-2) :26-34
[3]  
Bergson C, 1995, J NEUROSCI, V15, P7821
[4]   Glutamate-triggered events inducing corticostriatal long-term depression [J].
Calabresi, P ;
Centonze, D ;
Gubellini, P ;
Marfia, GA ;
Bernardi, G .
JOURNAL OF NEUROSCIENCE, 1999, 19 (14) :6102-6110
[5]   MOLECULAR DIVERSITY OF THE DOPAMINE-RECEPTORS [J].
CIVELLI, O ;
BUNZOW, JR ;
GRANDY, DK .
ANNUAL REVIEW OF PHARMACOLOGY AND TOXICOLOGY, 1993, 33 :281-307
[6]  
De la Garza R, 2000, SYNAPSE, V37, P232, DOI 10.1002/1098-2396(20000901)37:3<232::AID-SYN7>3.0.CO
[7]  
2-7
[8]   Distribution of D4 dopamine receptor in rat brain with sequence-specific antibodies [J].
Defagot, MC ;
Malchiodi, EL ;
Villar, MJ ;
Antonelli, MC .
MOLECULAR BRAIN RESEARCH, 1997, 45 (01) :1-12
[9]  
Defagot MC, 2000, J NEUROSCI RES, V59, P202, DOI 10.1002/(SICI)1097-4547(20000115)59:2<202::AID-JNR6>3.0.CO
[10]  
2-5