Lys13 plays a crucial role in the functional adaptation of the thermophilic triose-phosphate isomerase from Bacillus stearothermophilus to high temperatures

The thermophilic triose-phosphate isomerases (TIMs) of Bacillus stearothermophilus (bTIM) and Thermotoga maritima (tTIM) have been found to possess a His(12)- Lys(13) pair instead of the Asn(12)-Gly(13) pair normally present in mesophilic TIMs. His(12) in bTIM was proposed to prevent deamidation at high temperature, while the precise role of Lys(13) is unknown. To investigate the role of the His(12) and Lys(13) pair in the enzyme's thermoadaptation, we reintroduced the "mesophilic residues" Asn and Gly into both thermophilic TIMs. Neither double mutant displayed diminished structural stability, but the bTIM double mutant showed drastically reduced catalytic activity. No similar behavior was observed with the tTIM double mutant, suggesting that the presence of the His(12) and Lys(13) cannot be systematically correlated to thermoadaptation in TIMs, We determined the crystal structure of the bTIM double mutant complexed with 2-phosphoglycolate to 2,4-Angstrom resolution. A molecular dynamics simulation showed that upon substitution of Lys(13) to, Gly an increase of the flexibility of loop 1 is observed, causing an incorrect orientation of the catalytic Lys(10). This suggests that Lys(13) in bTIM plays a crucial role in the functional adaptation of this enzyme to high temperature. Analysis of bTIM single mutants supports this assumption.