Differentiation between Mycobacterium farcinogenes and Mycobacterium senegalense strains based on 16S-23S ribosomal DNA internal transcribed spacer sequences

被引：34

作者：

Hamid, ME

Roth, A

Landt, O

Kroppenstedt, RM

Goodfellow, M ^{[1
]}

Mauch, H

机构：

[1] Univ Newcastle Upon Tyne, Dept Agr & Environm Sci, Newcastle Upon Tyne NE1 7RU, Tyne & Wear, England

[2] Univ Khartoum, Fac Vet Sci, Dept Prevent Med & Publ Hlth, Khartoum N, Sudan

[3] Lungenklin Heckeshorn, Inst Mikrobiol & Immunol, D-14109 Berlin, Germany

[4] TIB Molbiol, D-10829 Berlin, Germany

[5] Deutsch Sammlung Mikroorganismen & Zellkulturen G, D-38124 Braunschweig, Germany

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2002年 / 40卷 / 02期

基金：

英国惠康基金;

关键词：

D O I：

10.1128/JCM.40.2.707-711.2002

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

16S ribosomal DNA (rDNA) and 16S-23S internal transcribed spacer rDNA sequence analyses were performed on Mycobacterium farcinogenes and H. senegalense strains and 26 strains of other rapidly growing mycobacteria to investigate the phylogenetic structure of bovine farcy mycobacteria within the M. fortuitum complex. M. farcinogenes and M. senegalense were indistinguishable in their 5'-end 16S rDNA but showed both considerable interspecies spacer sequence divergence and a high level of intraspecies sequence stability. A rapid detection assay using PCR and hybridization with species-specific probes was developed. The assay was specific among 46 species other than M. farcinogenes and M. senegalense and correctly identified all M. farcinogenes and M. senegalense strains. PCR- and 16S-23S rDNA sequence-based detection will be a valuable approach for diagnosis of the causal agents of African bovine farcy in cattle.

引用

页码：707 / 711

页数：5

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