A competitive immunoassay to detect a hapten using an enzyme-labelled peptide mimotope as tracer

被引：12

作者：

Sellrie, F

Schenk, JA

Behrsing, O

Böttger, V

Micheel, B

机构：

[1] Univ Potsdam, Inst Biochem & Biol, D-14476 Golm, Germany

[2] Max Delbruck Ctr Mol Med, D-13125 Berlin, Germany

[3] Wilex Biotechnol GMBH, D-81675 Munich, Germany

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 2002年 / 261卷 / 1-2期

关键词：

mimotope; enzyme conjugate; competitive immunoassay; fluorescein;

D O I：

10.1016/S0022-1759(01)00561-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Mimotope peptides-peptides which mimic the binding of a hapten to its corresponding monoclonal antibody-were conjugated to peroxidase and used in competitive immunoassay. The established immunoassay was used to quantitatively determine the concentration of hapten. As model system in all the experiments described here, we used the binding of the monoclonal antibody B13-DE1 to fluorescein and the corresponding peptide mimotope. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：141 / 144

页数：4

共 12 条

[1]

Hermanson G. T., 1996, BIOCONJUGATE TECHNIQ

[2] Cutting edge: DNA immunization with minigenes of carbohydrate mimotopes induce functional anti-carbohydrate antibody response [J].