PspE (phage-shock protein E) os Escherichia coli is a rhodanese

被引:48
作者
Adams, H
Teertstra, W
Koster, M
Tommassen, J
机构
[1] Univ Utrecht, Dept Mol Microbiol, NL-3584 CH Utrecht, Netherlands
[2] Univ Utrecht, Biomembrane Inst, NL-3584 CH Utrecht, Netherlands
关键词
PspE; phage-shock response; rhodanese; Escherichia coli;
D O I
10.1016/S0014-5793(02)02695-9
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The psp (phage-shock protein) operon of Eschevichia coli is induced when the bacteria are infected by filamentous phage and under several other stress conditions. The physiological role of the individual Psp proteins is still not known. We demonstrate here that the last gene of the operon, pspE, encodes a thiosulfate: cyanide sulfurtransferase (EC 2.8.1.1; rhodanese). Kinetic analysis revealed that catalysis occurs via a double displacement mechanism as described for other rhodaneses. The K(m)s for SSO32- and CN- were 4.6 and 27 mM, respectively. (C) 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.
引用
收藏
页码:173 / 176
页数:4
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