Detection of the metastatic potential of blood-borne and immunomagnetically enriched epithelial cells by quantitative erbB-2 RT-PCR

被引：7

作者：

Brandt, B ^{[1
]}

Griwatz, C ^{[1
]}

Heidl, S ^{[1
]}

Assmann, G ^{[1
]}

Zanker, KS ^{[1
]}

机构：

[1] UNIV WITTEN HERDECKE,INST IMMUNOL,WITTEN,GERMANY

来源：

CLINICAL & EXPERIMENTAL METASTASIS | 1996年 / 14卷 / 04期

关键词：

adjuvant therapy; blood-borne epithelial cells; hematogenous metastasis; mRNA c-erbB-2 dosage; quantitative competitive RT-PCR;

D O I：

10.1007/BF00123399

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

A quantitative competitive RT-PCR targeting the specific mRNA of c-erbB-2 is described. It is well known that in many cancers the c-erbB-2 gene dosage is elevated and/or the mRNA is overexpressed leading to a poor prognosis. The recently established method to enrich epithelial cells from peripheral blood made it reasonable and logical to develop a sophisticated competitive RT-PCR method to estimate the c-erbB-2 mRNA load of such cells. By this enrichment method it was discovered that cancer cells can be either isolated as single cells or clusters from patients' blood. The plausibility for their metastatic potential is derived from the measurement of the c-erbB-2 mRNA expression. A SKBR3 cell model suggested that the c-erbB-2 mRNA load is between 168 and 336 molecules per cell. Results of the quantitative competitive RT-PCR method can be used as a rational to determine the efficiency of anti-oncogenic therapies and as a criterion in adjuvant therapy decision.

引用

页码：399 / 408

页数：10

共 24 条

[1] GENE DIAGNOSTICS PROVIDE NEW INSIGHTS INTO BREAST-CANCER PROGNOSIS AND THERAPY [J].