Cell cycle and morphological alterations as indicative of apoptosis promoted by UV irradiation in S-cerevisiae

被引：74

作者：

Del Carratore, R

Della Croce, C

Simili, M

Taccini, E

Scavuzzo, M

Sbrana, S

机构：

[1] CNR, Ist Mutagenesis & Differenziat, I-56124 Pisa, Italy

[2] Univ Pisa, Dept Allergy & Clin Immunol, Pisa, Italy

[3] Vet Med Univ, Dept Anim Pathol, Pisa, Italy

来源：

MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS | 2002年 / 513卷 / 1-2期

关键词：

apoptosis; yeast; DNA damage; cytofluorimetry;

D O I：

10.1016/S1383-5718(01)00310-2

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

An apoptotic phenotype induced by oxygen radicals or Bax expression has been observed in Saccharomyces cerevisiae yeast cells by electron and fluorescence microscopy. In this work, we analyzed DNA content and cellular morphology of S. cerevisiae after H2O2 or UV treatment by TdT-mediated dUTP nick end labeling (TUNEL)-test and flow cytofluorimetry. A TUNEL-positive phenotype was observed in both cases, on the same samples a dose-dependent increase in the sub-G, population was pointed out by flow cytometry. Sub-G, cells were isolated by flow sorting and analyzed by electron microscopy. This population showed condensed chromatin in the nucleus and cell shrinking. This paper reports the first evidence of apoptosis in yeast cells induced by DNA damage after UV irradiation. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：183 / 191

页数：9

共 34 条

[1] The origin of programmed cell death [J].