Assessing Methods for Blood Cell Cytotoxic Responses to Inorganic Nanoparticles and Nanoparticle Aggregates

被引：151

作者：

Diaz, Belen ^{[5
]}

Sanchez-Espinel, Christian ^{[5
]}

Arruebo, Manuel ^{[1
]}

Faro, Jose ^{[2
,5
]}

de Miguel, Encarnacio ^{[3
]}

Magadan, Susana ^{[4
]}

Yaguee, Clara ^{[1
]}

Fernandez-Pacheco, Rodrigo ^{[1
]}

Ibarra, M. Ricardo ^{[1
]}

Santamaria, Jesus ^{[1
]}

Gonzalez-Fernandez, Africa ^{[5
]}

机构：

[1] Univ Zaragoza, INA, E-50009 Zaragoza, Spain

[2] Gulbenkian Inst Sci, Oeiras, Portugal

[3] Univ Vigo, Fac Biol, Cellular Biol Area, Vigo 36310, Pontevedra, Spain

[4] Inst Super Saude Alto Ave ISAVE, P-483031 Geraz Do Minho, Pvl, Portugal

[5] Univ Vigo, Fac Biol, Immunol Area, Vigo 36310, Pontevedra, Spain

来源：

SMALL | 2008年 / 4卷 / 11期

关键词：

cytotoxicity; magnetic nanopartictes; reactive oxygen species; silica;

D O I：

10.1002/smll.200800199

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Inorganic nanoparticles (NPs) show great potential for medicinal therapy. However, biocompatibility studies are essential to determine if they are safe. Here, five different NPs are compared for their cytotoxicity, internalization, aggregation in medium, and reactive oxygen species (ROS) production, using tumoral and normal human blood cells. Differences depending on the cell type are analyzed, and no direct correlation between ROS production and cell toxicity is found. Results are discussed with the aim of standardizing the procedures for the evaluation of the toxicity.

引用

页码：2025 / 2034

页数：10

共 40 条

[1] Determination of the total concentration and speciation of Al(III) in tea infusions [J].

Alberti, G ;

Biesuz, R ;

Profumo, A ;

Pesavento, M .

JOURNAL OF INORGANIC BIOCHEMISTRY, 2003, 97 (01) :79-88

[2] Antibody-functionalized hybrid superparamagnetic nanoparticles [J].

Arruebo, Manuel ;

Fernandez-Pacheco, Rodrigo ;

Velasco, Beatriz ;

Marquina, Clara ;

Arbiol, Jordi ;

Irusta, Silvia ;

Ibarra, M. Ricardo ;

Santamaria, Jesus .

ADVANCED FUNCTIONAL MATERIALS, 2007, 17 (09) :1473-1479

[3] Magnetic nanoparticles for drug delivery [J].

Arruebo, Manuel ;

Fernandez-Pacheco, Rodrigo ;

Ibarra, M. Ricardo ;

Santamaria, Jesus .

NANO TODAY, 2007, 2 (03) :22-32

[4] Sustained release of doxorubicin from zeolite-magnetite nanocomposites prepared by mechanical activation [J].

Arruebo, Manuel ;

Fernandez-Pacheco, Rodrigo ;

Irusta, Silvia ;

Arbiol, Jordi ;

Ibarra, M. Ricardo ;

Santamaria, Jesus .

NANOTECHNOLOGY, 2006, 17 (16) :4057-4064

[5] Influence of shape, adhesion and simulated lung mechanics on amorphous silica nanoparticle toxicity [J].

Brown, Scott C. ;

Kamal, Mohammed ;

Nasreen, Najmunnisa ;

Baumuratov, Aidos ;

Sharma, Parvesh ;

Antony, Veena B. ;

Moudgil, Brij M. .

ADVANCED POWDER TECHNOLOGY, 2007, 18 (01) :69-79

[6] The effect of surface charge on the uptake and biological function of mesoporous silica nanoparticles 3T3-L1 cells and human mesenchymal stem cells [J].

Chung, Tsai-Hua ;

Wu, Si-Han ;

Yao, Ming ;

Lu, Chen-Wen ;

Lin, Yu-Shen ;

Hung, Yann ;

Mou, Chung-Yuan ;

Chen, Yao-Chang ;

Huang, Dong-Ming .

BIOMATERIALS, 2007, 28 (19) :2959-2966

[7]

De Teresa JM, 2005, INT J NANOTECHNOL, V2, P3

[8] Pure-silica zeolites (porosils) as model solids for the evaluation of the physicochemical features determining silica toxicity to macrophages [J].

Fenoglio, I ;

Croce, A ;

Di Renzo, F ;

Tiozzo, R ;

Fubini, B .

CHEMICAL RESEARCH IN TOXICOLOGY, 2000, 13 (06) :489-500

[9] The cell cycle related apoptotic susceptibility to arsenic trioxide is associated with the level of reactive oxygen species [J].

Gao, F ;

Yi, J ;

Yuan, JQ ;

Shi, GY ;

Tang, XM .

CELL RESEARCH, 2004, 14 (01) :81-85

[10] BIOCHEMICAL AND BIOLOGICAL CHARACTERIZATION OF LYMPHOCYTE REGULATORY MOLECULES .5. IDENTIFICATION OF AN INTERLEUKIN 2-PRODUCING HUMAN-LEUKEMIA T-CELL LINE [J].

GILLIS, S ;

WATSON, J .

JOURNAL OF EXPERIMENTAL MEDICINE, 1980, 152 (06) :1709-1719

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