Isolation and characterization of a cDNA clone encoding an osmotin-like protein from Arabidopsis thaliana

被引：39

作者：

Capelli, N ^{[1
]}

Diogon, T ^{[1
]}

Greppin, H ^{[1
]}

Simon, P ^{[1
]}

机构：

[1] UNIV GENEVA, LAB BIOCHIM & PHYSIOL VEGETALES, CH-1211 GENEVA 4, SWITZERLAND

来源：

GENE | 1997年 / 191卷 / 01期

关键词：

pathogenesis-related protein; osmotic stress-related protein; thaumatin-like protein; THAUMATIN-LIKE PROTEIN; NUCLEOTIDE-SEQUENCE; TOBACCO; ADAPTATION; EXPRESSION; POTATO; CELLS;

D O I：

10.1016/S0378-1119(97)00029-2

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A phage library of cDNA from Arabidopsis thaliana has been screened with oligodeoxyribonucleotides designed from regions of high homology found in tobacco osmotin and other osmotin-like proteins. One of the selected clones, Atosm34, presents a 734 bp open reading frame encoding a polypeptide of 244 amino acids, including the putative N-terminal signal and C-terminal propeptide sequences. Comparative alignment reveals extensive homologies to osmotin and the osmotin-like proteins found in Solanaceae, and also to a related polypeptide found in soybean. Genomic hybridization suggests that the cDNA obtained here corresponds to a single copy gene, and RNA blot analysis showed that the level of expression is highest in old leaves. (C) 1997 Elsevier Science B.V.

引用

页码：51 / 56

页数：6

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