Trophoblast invasion of spiral arteries: A novel in vitro model

被引:86
作者
Cartwright, JE
Kenny, LC
Dash, PR
Crocker, IP
Aplin, JD
Baker, PN
Whitley, GSJ
机构
[1] St George Hosp, Sch Med, Dept Biochem & Immunol, London SW17 0RE, England
[2] St Marys Hosp, Maternal & Fetal Hlth Res Ctr, Manchester M13 0JH, Lancs, England
基金
英国惠康基金;
关键词
D O I
10.1053/plac.2001.0760
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Extravillous trophoblasts invade the uterine all (interstitial invasion) and the spiral arteries (endovascular invasion), replacing the cells of the vessel wall and creating a high-flow low-resistance vessel. We have developed a novel model to allow the interactions between the invading trophoblast cells and the cells of the spiral artery to be directly examined. Unmodified (non-placental bed) spiral arteries were obtained from uterine biopsies at caesarean section. Fluorescently labelled trophoblasts were seeded on top of artery segments embedded in fibrin gels (to study interstitial invasion) or perfused into the lumen of arteries mounted on a pressure myograph (to study endovascular invasion). Trophoblasts were incubated with the vessels for 3-5 days prior to cryo-sectioning. Both interstitial and endovascular interactions/invasion could clearly be detected and a comparison of the extravillous trophoblast cell line, SGHPL-4 and primary first trimester cytotrophoblasts showed both to be invasive in this model. This novel method will prove useful in an area where in vitro studies have been hampered by the lack of suitable models directly examining cellular interactions during invasion. (C) 2002 Elsevier Science Ltd.
引用
收藏
页码:232 / 235
页数:4
相关论文
共 23 条
[1]  
Blankenship TN, 1997, ACTA ANAT, V158, P227
[2]   TROPHOBLASTIC INVASION AND MODIFICATION OF UTERINE VEINS DURING PLACENTAL DEVELOPMENT IN MACAQUES [J].
BLANKENSHIP, TN ;
ENDERS, AC ;
KING, BF .
CELL AND TISSUE RESEARCH, 1993, 274 (01) :135-144
[3]   Hepatocyte growth factor regulates human trophoblast motility and invasion: a role for nitric oxide [J].
Cartwright, JE ;
Holden, DP ;
Whitley, GS .
BRITISH JOURNAL OF PHARMACOLOGY, 1999, 128 (01) :181-189
[4]  
Choy M Y, 2000, Early Pregnancy, V4, P124
[5]   The phagocytic activity of human first trimester extravillous trophoblast [J].
Choy, MY ;
Manyonda, IT .
HUMAN REPRODUCTION, 1998, 13 (10) :2941-2949
[6]   Decidual spiral artery remodelling begins before cellular interaction with cytotrophoblasts [J].
Craven, CM ;
Morgan, T ;
Ward, K .
PLACENTA, 1998, 19 (04) :241-252
[7]   BETA-1 INTEGRINS IN 3RD-TRIMESTER HUMAN PLACENTAE - NO DIFFERENTIAL EXPRESSION IN PATHOLOGICAL PREGNANCY [J].
DIVERS, MJ ;
BULMER, JN ;
MILLER, D ;
LILFORD, RJ .
PLACENTA, 1995, 16 (03) :245-260
[8]   EARLY STAGES OF TROPHOBLASTIC INVASION OF THE MATERNAL VASCULAR SYSTEM DURING IMPLANTATION IN THE MACAQUE AND BABOON [J].
ENDERS, AC ;
KING, BF .
AMERICAN JOURNAL OF ANATOMY, 1991, 192 (04) :329-346
[9]   Hypoxia alters early gestation human cytotrophoblast differentiation invasion in vitro and models the placental defects that occur in preeclampsia [J].
Genbacev, O ;
Joslin, R ;
Damsky, CH ;
Polliotti, BM ;
Fisher, SJ .
JOURNAL OF CLINICAL INVESTIGATION, 1996, 97 (02) :540-550
[10]   The role of trophoblast in the physiological change in decidual spiral arteries [J].
Kam, EPY ;
Gardner, L ;
Loke, YW ;
King, A .
HUMAN REPRODUCTION, 1999, 14 (08) :2131-2138