The use of autologous enriched bone marrow MSCs to enhance osteoporotic bone defect repair in long-term estrogen deficient goats

被引：127

作者：

Cao, Lei ^{[1
,4
]}

Liu, Guangwang ^{[1
]}

Gan, Yaokai ^{[1
]}

Fan, Qiming ^{[1
]}

Yang, Fei ^{[1
]}

Zhang, Xiaoling ^{[2
,3
]}

Tang, Tingting ^{[1
]}

Dai, Kerong ^{[1
,2
,3
]}

机构：

[1] Shanghai Jiao Tong Univ, Sch Med, Shanghai Peoples Hosp 9, Shanghai Key Lab Orthoped Implant,Dept Orthoped, Shanghai 200011, Peoples R China

[2] Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Hlth Sci, Orthoped Cellular & Mol Biol Lab, Shanghai 200025, Peoples R China

[3] Shanghai Jiao Tong Univ, Sch Med, Shanghai 200025, Peoples R China

[4] Shanghai Jiao Tong Univ, Shanghai Peoples Hosp 1, Shanghai 200080, Peoples R China

来源：

BIOMATERIALS | 2012年 / 33卷 / 20期

关键词：

Enrichment; Stem cells; Osteoporotic; Bone defect; BETA-TRICALCIUM PHOSPHATE; STROMAL CELLS; STEM-CELLS; OVARIECTOMIZED EWES; IN-VIVO; MODEL; SHEEP; RAT; DIFFERENTIATION; GENDER;

D O I：

10.1016/j.biomaterials.2012.03.069

中图分类号：

R318 [生物医学工程];

学科分类号：

100103 [病原生物学];

摘要：

Bone defects are common in elderly patients suffering from osteoporosis. Current methods of bone defect treatment for osteoporosis are not always satisfactory. In this study, we demonstrated that bone marrow mesenchymal stem cells (MSCs) harvested from goats with long-term estrogen deficiencies exhibited a lower proliferation rate and decreased osteogenic capacity, which are critical obstacles for bone defect repair in the elderly. However, by combining autologous enriched bone marrow mesenchymal stem cells with porous beta-TCP, we successfully repaired critical-sized bone defects in the medial femoral condyle of the osteoporotic goats. Both micro-CT images and histomorphometry analysis illustrated improved bone formation following the enriched MSC therapy. Thus, we proposed autologous enriched bone marrow mesenchymal stem cells as a quick, safe therapeutic strategy to treat osteoporotic bone defects. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：5076 / 5084

页数：9

共 40 条

[1]

Bergman RJ, 1996, J BONE MINER RES, V11, P568

[2]

Effects of a new selective estrogen receptor modulator (MDL 103,323) on cancellous and cortical bone in ovariectomized ewes: A biochemical, histomorphometric, and densitometric study [J].