Methylation-specific sequencing of GSTP1 gene promoter in circulating/extracellular DNA from blood and urine of healthy donors and prostate cancer patients

被引：52

作者：

Bryzgunova, Olga E. ^{[1
]}

Morozkin, Evgeniy S. ^{[1
]}

Yarmoschuk, Sergey V. ^{[2
]}

Vlassov, Valentin V. ^{[1
]}

Laktionov, Pavel P. ^{[1
]}

机构：

[1] Russian Acad Sci, Inst Chem Biol & Fundamental Med, Siberian Div, Novosibirsk 630090, Russia

[2] Municipal Clin Hosp N 1, Novosibirsk, Russia

来源：

CIRCULATING NUCLEIC ACIDS IN PLASMA AND SERUM V | 2008年 / 1137卷

基金：

俄罗斯基础研究基金会;

关键词：

circulating DNA; extracellular DNA; cell-surface-bound DNA; methylation of GSTP1 gene; urine; prostate cancer;

D O I：

10.1196/annals.1448.039

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Hypermethylated promoters of cancer-related genes represent convenient targets for early cancer diagnosis and monitoring based on circulating/extracellular DNA (cir/exDNA) from human blood and urine. The frequency of detection of methylated tumor suppressor genes in plasma or urine samples is usually lower than in the samples of tumor tissue because of a low concentration of target DNA and potential polymorphism of cirDNA methylation. Sequencing of the methylated cir/exDNA of tumor suppression genes provides information about methylation of the cirDNA originating from the tumor cells, which is necessary for optimization of cancer diagnosis. In this work, by sequencing chemically converted cir/exDNA, we have studied the cytosine methylation profile of GSTP1 gene promoter (1001-1302, X08058) in the pool of cir/exDNA from the blood and urine of healthy men, prostate cancer (PCa) patients, and patients with benign prostatic hyperplasia (BPH). We demonstrated that the data on cir/exDNA methylation could be obtained from sequencing of the cir/exDNA from blood and urine. The DNA isolated from blood plasma and the eluates of blood cells and urine of each patient were characterized by the same methylation profile of the GSTP1 gene. The profile of GSTP1 gene methylation in the extracellular DNA of PCa patients differs from the profiles characteristic of healthy donors and patients with BPH.

引用

页码：222 / 225

页数：4

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