How to characterize meiotic functions in plants?

被引：18

作者：

Mercier, R ^{[1
]}

Grelon, M ^{[1
]}

Vezon, D ^{[1
]}

Horlow, C ^{[1
]}

Pelletier, G ^{[1
]}

机构：

[1] INRA, Genet & Ameliorat Plantes Stn, F-78026 Versailles, France

来源：

BIOCHIMIE | 2001年 / 83卷 / 11-12期

关键词：

Arabidopsis thaliana; meiosis; forward genetics; reverse genetics;

D O I：

10.1016/S0300-9084(01)01348-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Our understanding of plant meiosis is rapidly increasing thanks to the model Arabidopsis thaliana. Here we present the results of a screening for meiotic mutants carried out with a library containing 30 719 T-DNA insertion lines. An average of one mutant per 1000 lines was recovered. Several phenotypic classes could be distinguished and are presented. In parallel, 39 proteins known to be involved in meiosis in non-plant organisms were chosen and a search was performed for homologue sequences in the completed Arabidopsis thaliana genome. Approximately 30% of the meiotic related sequences showed similarities with one or several Arabidopsis putative genes. The relevance of forward versus reverse genetics in order to characterize meiotic functions is discussed. (C) 2001 Societe francaise de biochimie et biologic moleculaire / Editions scientifiques et medicales Elsevier SAS. All rights reserved.

引用

页码：1023 / 1028

页数：6

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