Reflex: intramolecular barcoding of long-range PCR products for sequencing multiple pooled DNAs

被引：1

作者：

Casbon, James A. ^{[1
]}

Slatter, Andrew F. ^{[1
]}

Musgrave-Brown, Esther ^{[1
]}

Osborne, Robert J. ^{[1
]}

Lichtenstein, Conrad P. ^{[1
]}

Brenner, Sydney ^{[1
,2
]}

机构：

[1] Populat Genet Technol Ltd, Babraham Inst, Babraham CB22 3AT, Cambs, England

[2] Kings Coll London, Cambridge CB2 1ST, England

来源：

NUCLEIC ACIDS RESEARCH | 2013年 / 41卷 / 10期

基金：

英国惠康基金;

关键词：

D O I：

10.1093/nar/gkt228

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

We present an intramolecular reaction, Reflex (TM), to derive shorter, sequencer-ready, daughter polymerase chain reaction products from a pooled population of barcoded long-range polymerase chain reaction products, whilst still preserving the cognate DNA barcodes. Our Reflex workflow needs only a small number of primer extension steps to rapidly enable uniform sequence coverage of long contiguous sequence targets in large numbers of samples at low cost on desktop next-generation sequencers.

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