Chromosome landing at the tomato Bs4 locus

被引：14

作者：

Ballvora, A

Schornack, S

Baker, BJ

Ganal, M

Bonas, U

Lahaye, T

机构：

[1] Univ Halle Wittenberg, Inst Genet, D-06120 Halle Saale, Saale, Germany

[2] CNRS, Inst Sci Vegetales, F-91190 Gif Sur Yvette, France

[3] Univ Calif Berkeley, USDA, Ctr Plant Gene Express, Albany, CA 94710 USA

[4] Univ Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA 94720 USA

[5] Inst Plant Genet & Crop Plant Res, D-06466 Gatersleben, Germany

来源：

MOLECULAR GENETICS AND GENOMICS | 2001年 / 266卷 / 04期

关键词：

avrBs3; avrBs3-2; sequence-tagged-site (STS) marker; bacterial spot disease; cleaved amplified polymorphic sequence (CAPS);

D O I：

10.1007/s004380100583

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The tomato (Lycopersicon esculentum) Bs4 gene confers resistance to strains of Xanthomonas campestris pathovar vesicatoria that express the avirulence protein AvrBs4. As part of a map-based cloning strategy for the isolation of Bs4, we converted Bs4-linked amplified fragment length polymorphism (AFLP) and restriction fragment length polymorphism (RFLP) markers into locus-specific sequence-tagged-site (STS) markers. The use of these markers for the analysis of 1972 meiotic events allowed high-resolution genetic mapping within a 1.2-cM interval containing the target gene. Two tomato yeast artificial chromosome (YAC clones, each harboring inserts of approximately 250 kb, were identified using the marker most closely linked to Bs4. YAC end-specific markers were established and employed to construct a local YAC contig. The ratio of physical to genetic distance at Bs4 was calculated to be 280 kb/cM, revealing that recombination rates in this region are about three times higher than the genome-wide average. Mapping of YAC end-derived markers demonstrated that the Bs4 locus maps within a region of 250 kb, corresponding to a genetic interval of 0.9 cM.

引用

页码：639 / 645

页数：7

共 57 条

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