Hydrophilic interaction chromatography on amino-silica phases complements reversed-phase high-performance chromatography and capillary electrophoresis for peptide analysis

被引：99

作者：

Oyler, AR

Armstrong, BL

Cha, JY

Zhou, MX

Yang, Q

Robinson, RI

Dunphy, R

Burinsky, DJ

机构：

[1] R.W. Johnson Pharmaceutical Res. I., Analytical R and D Division, Raritan, NJ 08869-0602

来源：

JOURNAL OF CHROMATOGRAPHY A | 1996年 / 724卷 / 1-2期

关键词：

hydrophilic interaction chromatography; capillary electrophoresis; liquid chromatography mass spectrometry; stationary phases; LC; diastereomer separation; peptides; atosiban;

D O I：

10.1016/0021-9673(95)00987-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Hydrophilic interaction chromatography (HILIC) on amine bonded-phase silica columns provides separations of peptides that are complementary to those obtained with reversed-phase HPLC and free solution capillary electrophoresis. This is illustrated with the peptide drug atosiban and nine diastereomers. Moreover, one of the HILIC methods was suitable for coupling with electrospray mass spectrometry.

引用

页码：378 / 383

页数：6

共 15 条

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