Functional expression of the fractalkine (CX3C) receptor and its regulation by lipopolysaccharide in rat microglia

被引：63

作者：

Boddeke, EWGH

Meigel, I

Frentzel, S

Biber, K

Renn, LQ

Gebicke-Härter, P

机构：

[1] Univ Groningen, Dept Med Physiol, NL-9712 KZ Groningen, Netherlands

[2] Novartis Pharmaceut, NS Res, CH-4002 Basel, Switzerland

[3] Univ Freiburg, Dept Psychiat, D-79104 Freiburg, Germany

来源：

EUROPEAN JOURNAL OF PHARMACOLOGY | 1999年 / 374卷 / 02期

关键词：

fractalkine; CX3C1; receptor; lipopolysaccharide;

D O I：

10.1016/S0014-2999(99)00307-6

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Functional expression of CX,CRI, a recently discovered receptor for the chemokine fractalkine, was investigated in cultured rat microglia. Reverse transcriptase polymerase chain reaction (PCR) experiments show abundant expression of fractalkine receptor mRNA in microglia. mRNA expression of fractalkine was undetectable in astrocytes and microglia but was very strong in cortical neurons Incubation of microglia with lipopolysaccharide (100 ng/ml) transiently suppressed expression of fractalkine receptor mRNA. Fractalkine induced a concentration-dependent (10(-10)-10(-8) M) and, at high concentrations, oscillatory mobilization of intracellular Ca2+ in microglia The concentration-response curve of fractalkine was shifted to the right after 12 h incubation with lipopolysaccharide. It is concluded that treatment with endotoxin downregulates expression of fractalkine receptor mRNA in rat microglia and suppresses the functional response to fractalkine. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：309 / 313

页数：5

共 17 条

[1] Chemokines and leukocyte traffic [J].