Whole blood assay to access T cell-immune responses to Mycobacterium tuberculosis antigens in healthy Brazilian individuals

被引：7

作者：

Antas, PRZ

Cardoso, FLL

Oliveira, EB

Gomes, PKC

Cunha, KS

Sarno, EN

Sampaio, EP

机构：

[1] Fiocruz MS, Inst Oswaldo Cruz, Lab Hansenfase, BR-21045900 Rio De Janeiro, Brazil

[2] Hosp Municipal Raphael Paula & Souza, Rio De Janeiro, Brazil

来源：

MEMORIAS DO INSTITUTO OSWALDO CRUZ | 2004年 / 99卷 / 01期

关键词：

tuberculosis (TB); T cell-responses; whole blood; PPD; latent TB infection; interferon gamma;

D O I：

10.1590/S0074-02762004000100009

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

The production of interferon gamma (IFNgamma) guarantees effective T cell-mediated immunity against Mycobacterium tuberculosis infection. In the present study, we simply compare the in vitro immune responses to Mycobacterium antigens in terms of IFNgamma production in a total of 10 healthy Brazilian volunteers. Whole blood and mononuclear cells were cultivated in parallel with PPD, Ag85B, and M. bovis hsp65, and five-days supernatants were harvested for cytokine detection by ELISA. The inter-assay result was that the overall profile of agreement in response to antigens was highly correlated (r(2) = 0.9266; p = 0.0102). Potential analysis is in current progress to dictate the usefulness of this method to access the immune responses also in tuberculosis patients and its contacts.

引用

页码：53 / 55

页数：3

共 10 条

[1] Kinetics of T cell-activation molecules in response to Mycobacterium tuberculosis antigens [J].

Antas, PRZ ;

Oliveira, EB ;

Milagres, AS ;

Franken, KC ;

Ottenhoff, THM ;

Klatser, P ;

Sarno, EN ;

Sampaio, EP .

MEMORIAS DO INSTITUTO OSWALDO CRUZ, 2002, 97 (08) :1097-1099

[2] WHOLE-BLOOD CULTURE FOR MEASURING MITOGEN-INDUCED T-CELL PROLIFERATION PROVIDES SUPERIOR CORRELATIONS WITH DISEASE STATE AND T-CELL PHENOTYPE IN ASYMPTOMATIC HIV-INFECTED SUBJECTS [J].

BOCCHIERI, MH ;

TALLE, MA ;

MALTESE, LM ;

RAGUCCI, IR ;

HWANG, CC ;

GOLDSTEIN, G .

JOURNAL OF IMMUNOLOGICAL METHODS, 1995, 181 (02) :233-243

[3]

Brock I, 2001, INT J TUBERC LUNG D, V5, P462

[4] T-cell responses to the Mycobacterium tuberculosis- specific antigen ESAT-6 in Brazilian tuberculosis patients [J].

Cardoso, FLL ;

Antas, PRZ ;

Milagres, AS ;

Geluk, A ;

Franken, KLMC ;

Oliveira, EB ;

Teixeira, HC ;

Nogueira, SA ;

Sarno, EN ;

Klatser, P ;

Ottenhoff, THM ;

Sampaio, EP .

INFECTION AND IMMUNITY, 2002, 70 (12) :6707-6714

[5]

DELUCA PM, 2003, IN PRESS T R SOC TRO, V97

[6] Purification of His-tagged proteins by immobilized chelate affinity chromatography: The benefits from the use of organic solvent [J].

Franken, KLMC ;

Hiemstra, HS ;

van Meijgaarden, KE ;

Subronto, Y ;

den Hartigh, J ;

Ottenhoff, THM ;

Drijfhout, JW .

PROTEIN EXPRESSION AND PURIFICATION, 2000, 18 (01) :95-99

[7] Identification and characterization of the ESAT-6 homologue of Mycobacterium leprae and T-cell cross-reactivity with Mycobacterium tuberculosis [J].

Geluk, A ;

van Meijgaarden, KE ;

Franken, KLMC ;

Subronto, YW ;

Wieles, B ;

Arend, SM ;

Sampaio, EP ;

de Boer, T ;

Faber, WR ;

Naafs, B ;

Ottenhoff, THM .

INFECTION AND IMMUNITY, 2002, 70 (05) :2544-2548

[8]

Kochi A., 1991, TUBERCLE, V79, P71

[9] Comparison of a whole-blood interferon γ assay with tuberculin skin testing for detecting latent Mycobacterium tuberculosis infection [J].

Mazurek, GH ;

LoBue, PA ;

Daley, CL ;

Bernardo, J ;

Lardizabal, AA ;

Bishai, WR ;

Iademarco, MF ;

Rothel, JS .

JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION, 2001, 286 (14) :1740-1747

[10] T cell mediated immunity to Mycobacterium tuberculosis [J].

Stenger, S ;

Modlin, RL .

CURRENT OPINION IN MICROBIOLOGY, 1999, 2 (01) :89-93

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