PHA Productivity and Yield of Ralstonia eutropha When Intermittently or Continuously Fed a Mixture of Short Chain Fatty Acids

被引:11
作者
Chakraborty, Panchali [1 ]
Muthukumarappan, Kasiviswanathan [1 ]
Gibbons, William R. [2 ]
机构
[1] S Dakota State Univ, Dept Agr & Biosyst Engn, Brookings, SD 57006 USA
[2] S Dakota State Univ, Dept Biol & Microbiol, Brookings, SD 57006 USA
来源
JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY | 2012年
关键词
FED-BATCH CULTURE; POLYHYDROXYALKANOATE PRODUCTION; MICROBIAL-PRODUCTION; WAUTERSIA-EUTROPHA; ESCHERICHIA-COLI; ACETIC-ACID; POLY(3-HYDROXYBUTYRATE); BIOSYNTHESIS; INHIBITION; GLUCOSE;
D O I
10.1155/2012/506153
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The research described in this present study was part of a larger effort focused on developing a dual substrate, dual fermentation process to produce Polyhydroxyalkanoate (PHA). The focus of this study was developing and optimizing a strategy for feeding a mixture of SCFAs (simulated ARF) and maximizing PHA production in a cost-effective way. Three different feeding strategies were examined in this study. The substrate evaluated in this study for the growth phase of R. eutropha was condensed corn solubles, a low-value byproduct of the dry-mill, corn ethanol industry. The culture was grown to high cell densities in nitrogen-supplemented condensed corn solubles media in 5 L bioreactors. The overall growth rate of R. eutropha was 0.2 h(-1). The 20mL ARF feeding every 3 h from 48 to 109 h strategy gave the best results in terms of PHA production. PHA productivity (0.0697 g L-1 h(-1)), PHA concentration (8.37 g L-1), and PHA content (39.52%) were the highest when ARF was fed every 3 h for 61 h. This study proved that condensed corn solubles can be potentially used as a growth medium to boost PHA production by R. eutropha thus reducing the overall cost of biopolymer production.
引用
收藏
页数:8
相关论文
共 36 条
[1]   Production of poly(3-hydroxybutyrate) by fed-batch culture of recombinant Escherichia coli with a highly concentrated whey solution [J].
Ahn, WS ;
Park, SJ ;
Lee, SY .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2000, 66 (08) :3624-3627
[2]  
Amass W, 1998, POLYM INT, V47, P89, DOI 10.1002/(SICI)1097-0126(1998100)47:2<89::AID-PI86>3.0.CO
[3]  
2-F
[4]   RAPID GAS-CHROMATOGRAPHIC METHOD FOR DETERMINATION OF POLY-BETA-HYDROXYBUTYRIC ACID IN MICROBIAL BIOMASS [J].
BRAUNEGG, G ;
SONNLEITNER, B ;
LAFFERTY, RM .
EUROPEAN JOURNAL OF APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1978, 6 (01) :29-37
[6]  
Chakraborty P., 2009, J APPL MICROBIOL, V361, P1995
[7]   DETERMINATION OF POLY-BETA-HYDROXYBUTYRATE AND POLY-BETA-HYDROXYVALERATE IN ACTIVATED-SLUDGE BY GAS-LIQUID-CHROMATOGRAPHY [J].
COMEAU, Y ;
HALL, KJ ;
OLDHAM, WK .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1988, 54 (09) :2325-2327
[8]  
Crank M., 2004, Techno-economic Feasibility of Large-scale Production of Bio-based Polymers in Europe (PRO-BIP)
[9]   Green technology for conversion of food scraps to biodegradable thermoplastic polyhydroxyalkanoates [J].
Du, GC ;
Yu, J .
ENVIRONMENTAL SCIENCE & TECHNOLOGY, 2002, 36 (24) :5511-5516
[10]  
HANGGI UJ, 1990, NATO ADV SCI I E-APP, V186, P65